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Gctf specifications


Unique identifier OMICS_27330
Name Gctf
Software type Application/Script
Interface Command line interface
Restrictions to use None
Operating system Unix/Linux
Programming languages C
Parallelization CUDA
Computer skills Advanced
Version 1.18
Stability Stable
Maintained Yes


No version available


  • person_outline Kai Zhang

Publication for Gctf

Gctf citations


Structural basis for cofilin binding and actin filament disassembly

Nat Commun
PMCID: 5945598
PMID: 29749375
DOI: 10.1038/s41467-018-04290-w
call_split See protocol

[…] collected in two microscope sessions (414 and 697 micrographs, respectively). Image processing was performed with RELION 2.0 software,. After motion correction with MotionCorr and CTF estimation with Gctf, 1,093 images were selected for further image processing. Filaments were manually picked with e2helixboxer, after which 245,236 particles were extracted at a box size of 320 × 320 pixels. After 2 […]


Cryo EM reveals the structural basis of microtubule depolymerization by kinesin 13s

Nat Commun
PMCID: 5916938
PMID: 29695795
DOI: 10.1038/s41467-018-04044-8
call_split See protocol

[…] le decorated complexes image-movies were collected within a nominal defocus range of −0.5 to −1.5 µm. This resulted in a distribution of image defocus of −1.6 ± 0.8 µm (median ± SD) as estimated with Gctf. Individual NM-curved tubulin complexes were imaged at a higher defocus (−3.0 ± 0.5 µm). […]


Cryo EM structure of infectious bronchitis coronavirus spike protein reveals structural and functional evolution of coronavirus spike proteins

PLoS Pathog
PMCID: 5933801
PMID: 29684066
DOI: 10.1371/journal.ppat.1007009
call_split See protocol

[…] 4.5 μm after CTF estimation). The final image was bin-averaged to lead to a pixel size of 1.02 Å. The parameters of the microscope contrast transfer function were estimated for each micrograph using GCTF []. Particles were automatically picked and extracted using RELION [] with a box size of 320 pixels. Initially, ~802,000 particles were subjected to 2D alignment and clustering using RELION, and […]


Cryo EM structure of the RC LH core complex from an early branching photosynthetic prokaryote

Nat Commun
PMCID: 5908803
PMID: 29674684
DOI: 10.1038/s41467-018-03881-x
call_split See protocol

[…] We use Unblur for whole-frame motion correction and exposure weighting, Gctf for estimation of global and local contrast transfer function (CTF) parameters, Gautomatch (developed by Zhang K, MRC Laboratory of Molecular Biology, Cambridge, UK) for automatic particle pickin […]


RPAP3 provides a flexible scaffold for coupling HSP90 to the human R2TP co chaperone complex

Nat Commun
PMCID: 5902453
PMID: 29662061
DOI: 10.1038/s41467-018-03942-1

[…] As general methodology, MotionCorr2 was used for whole-frame motion correction, GCTF for estimation of the contrast transfer function parameters, and RELION-2.0 for all subsequent steps. Local motion was corrected in MotionCorr2 dividing the frame in 36 patches (6 × 6 patches), w […]


Ewald sphere correction using a single side band image processing algorithm

PMCID: 5862657
PMID: 29413409
DOI: 10.1016/j.ultramic.2017.11.001

[…] function fitting parameters are determined, just as it is for traditional CTF correction. Fortunately, the values obtained for defocus and astigmatism using established programs such as CTFFIND4 or GCTF are the same parameters needed for single sideband correction. The defocus given by CTFFIND4 or GCTF is what defines the particle origin in z for the single sideband phase correction just as it […]


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Gctf institution(s)
Medical Research Council Laboratory of Molecular Biology, Division of Structural Studies, Cambridge, UK
Gctf funding source(s)
Supported by the Medical Research Council, United Kingdom (MC_UP_A025_1011) and a Wellcome Trust New Investigator Award (WT100387).

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