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GenDecoder specifications

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Unique identifier OMICS_19575
Name GenDecoder
Interface Web user interface
Restrictions to use None
Input data An animal mitochondrial genome.
Computer skills Basic
Version 1.6
Stability Stable
Maintained No

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Publications for GenDecoder

GenDecoder citations

 (15)
library_books

Naturally acquired antibody responses to more than 300 Plasmodium vivax proteins in three geographic regions

2017
PMCID: 5614652
PMID: 28892517
DOI: 10.1371/journal.pntd.0005888

[…] layers. crude wgcf expressed bira (1 μl) was added to the translation layer. in vitro transcription and cell-free protein synthesis for the p. vivax protein library were carried out using the gendecoder 1000 robotic synthesizer (cellfree sciences) [, ]. based on previous studies, the wgcf system can synthesize biotinylated plasmodial protein ranging from 0.3 μg/ml to 26.5 μg/ml [,]. […]

library_books

Immunoscreening of Plasmodium falciparum proteins expressed in a wheat germ cell free system reveals a novel malaria vaccine candidate

2017
PMCID: 5380959
PMID: 28378857
DOI: 10.1038/srep46086

[…] wheat germ cell-free expressed bira (1 μl) was added to the translation layer. in vitro transcription and cell-free protein synthesis for the p. falciparum protein library were carried out using the gendecoder 1000 robotic synthesizer (cellfree sciences) as previously described. expression of the proteins was confirmed by western blotting using fitc- or hrp-conjugated streptavidin (thermofisher […]

library_books

H11/HSPB8 Restricts HIV 2 Vpx to Restore the Anti Viral Activity of SAMHD1

2016
PMCID: 4904303
PMID: 27379031
DOI: 10.3389/fmicb.2016.00883

[…] method was also described previously (, ; ). briefly, dna templates containing a biotin-ligating sequence (bls) were amplified by split-pcr using cdnas and corresponding primers, and then used in a gendecoder protein production system (cell free science, ehime, japan). for synthesis of hiv-2 vpx protein, vpx genes derived from the pgl-an proviral plasmid were generated by split-pcr and used […]

library_books

Establishment of a Wheat Cell Free Synthesized Protein Array Containing 250 Human and Mouse E3 Ubiquitin Ligases to Identify Novel Interaction between E3 Ligases and Substrate Proteins

2016
PMCID: 4889105
PMID: 27249653
DOI: 10.1371/journal.pone.0156718

[…] and 11 hect-type e3s, were prepared with a split-primer pcr method []., the recombinant e3 proteins were synthesized with the wheat cell-free system by using the robotic protein synthesizer gendecoder []. the expression of each e3 was checked with immunoblot analysis, and 250 of the 258 e3s were confirmed to be synthesized by the system ()., to establish the novel biochemical assay […]

library_books

Cytonuclear Interactions in the Evolution of Animal Mitochondrial tRNA Metabolism

2015
PMCID: 4558845
PMID: 26116918
DOI: 10.1093/gbe/evv124

[…] bachei, where the mitochondrial tga codon appears to have been recruited for an amino acid other than tryptophan (). comparative sequence analysis of the mt-genome from pl. bachei (nc_016697) using gendecoder () showed that 38% of tga codons in highly conserved positions (entropy < 1.0, gaps < 20%) correspond to serine in other animals, suggesting a novel reassignment of tga to serine […]

library_books

Novel Autoantigens Associated with Lupus Nephritis

2015
PMCID: 4476694
PMID: 26098692
DOI: 10.1371/journal.pone.0126564

[…] and 500 nm d-biotin (nacalai tesque, kyoto, japan) was added to both the translation and substrate layers []. in vitro transcription and cell-free protein synthesis for bpl were performed with the gendecoder 1000 robotic synthesizer (cellfree sciences)., the alphascreen assay (perkinelmer life and analytical sciences, boston, ma, usa) was carried out using a 384-well optiwell microtiter plate […]


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GenDecoder institution(s)
Departamento de Genética, Bioquímica e Inmunología, Facultad de Biología, Universidad de Vigo, Vigo, Spain

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