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- person_outline Genecopoeia Team
Macacine Herpesvirus 1 Antibody Prevalence and DNA Shedding among Invasive Rhesus Macaques, Silver Springs State Park, Florida, USA
[…] 4°C for 1 min, followed by 35 cycles of 98°C for 15 s, 60°C for 30 s, and 72°C for 60 s, with a final extension at 72°C for 10 min. We visualized PCR products on 2% agarose gels stained with RedView (Genecopoeia, Rockville, MD, USA). To verify the sequence of US5, we used AS9, AS2, AS8, and AS7 () to primer walk the region between the 3′ end of the US4 gene and the US6 gene. We purified amplicons […]
Fusarium graminearum: pathogen or endophyte of North American grasses?
[…] 72°C for 1 min and a final annealing at 72°C for 10 min. The PCR product (5 μl) was run on a 1% agarose gel containing either SYBRsafe (Thermo Fisher Scientific Inc., Waltham, MA, USA) or Greenview (GeneCopoeia Inc., Rockville, MD, USA) with 0.5 × TBE buffer for gel electrophoresis. The RPB2 PCR product, together with a 1‐kb ladder, used as a size standard, was visualized using ultraviolet light. […]
The novel ZIP4 regulation and its role in ovarian cancer
[…] per v0.0.13.2. Read mapping was performed by tophat2 v2.0.6 to the human genome hg19 (UCSC) with Gencode annotation v13 allowing no more than two mismatches. ZIP4 and PPARr CRISPR lentiVirus vectors (GeneCopoeia, Rockville, MD) were used for gene knockout transfected to 293T cells for virus packaging. PE01/PE04 and OVCAR3 cells were infected by virus 3 times and stable clones were selected by puro […]
Activation of TPA response element present in human Lemur Tyrosine Kinase 2 (lmtk2) gene increases its expression
[…] e element, we wanted to assess the effect of TPA on LMTK2 promoter activity. The LMTK2 promoter construct, driving the expression of a luciferase reporter gene (Gaussia luciferase), was obtained from Genecopoeia. As an expression control and for data normalization, the promoter plasmid also expressed secretory alkaline phosphatase (SEAP) under the constitutive control of a CMV promoter. This plasm […]
Molecular characterization of cell free eccDNAs in human plasma
[…] agen, Cat #150343) at 30 °C for 8 hours. The amplified products were purified using Agencourt AMPure XP (Beckman Coulter, A63881) and quantified using iQuant™ High Sensitivity dsDNA Quantitation Kit (GeneCopoeia, N011). […]
Silencing PRDM14 expression by an innovative RNAi therapy inhibits stemness, tumorigenicity, and metastasis of breast cancer
[…] Breast cancer cells were transfected with a HaloTag-PRDM14 lentivirus expression vector (GeneCopoeia). PRDM14-bound genomic DNA fragments were immunoprecipitated from cell lysates with HaloLink Resin (Promega, Madison, WI, USA) and immunoprecipitated with anti-H3K4me3, anti-H3K27me3, and […]
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