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Genecopoeia specifications

Information


Unique identifier OMICS_15905
Name Genecopoeia
Restrictions to use None
Community driven No
Data access Application programming interface
User data submission Not allowed
Maintained Yes

Maintainer


  • person_outline Genecopoeia Team

Genecopoeia citations

 (23)
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Macacine Herpesvirus 1 Antibody Prevalence and DNA Shedding among Invasive Rhesus Macaques, Silver Springs State Park, Florida, USA

2018
PMCID: 5782895
PMID: 29350146
DOI: 10.3201/eid2402.171439
call_split See protocol

[…] 4°C for 1 min, followed by 35 cycles of 98°C for 15 s, 60°C for 30 s, and 72°C for 60 s, with a final extension at 72°C for 10 min. We visualized PCR products on 2% agarose gels stained with RedView (Genecopoeia, Rockville, MD, USA). To verify the sequence of US5, we used AS9, AS2, AS8, and AS7 () to primer walk the region between the 3′ end of the US4 gene and the US6 gene. We purified amplicons […]

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Fusarium graminearum: pathogen or endophyte of North American grasses?

2017
New Phytol
PMCID: 5813145
PMID: 29160900
DOI: 10.1111/nph.14894
call_split See protocol

[…] 72°C for 1 min and a final annealing at 72°C for 10 min. The PCR product (5 μl) was run on a 1% agarose gel containing either SYBRsafe (Thermo Fisher Scientific Inc., Waltham, MA, USA) or Greenview (GeneCopoeia Inc., Rockville, MD, USA) with 0.5 × TBE buffer for gel electrophoresis. The RPB2 PCR product, together with a 1‐kb ladder, used as a size standard, was visualized using ultraviolet light. […]

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The novel ZIP4 regulation and its role in ovarian cancer

2017
Oncotarget
PMCID: 5685734
PMID: 29163813
DOI: 10.18632/oncotarget.21435
call_split See protocol

[…] per v0.0.13.2. Read mapping was performed by tophat2 v2.0.6 to the human genome hg19 (UCSC) with Gencode annotation v13 allowing no more than two mismatches. ZIP4 and PPARr CRISPR lentiVirus vectors (GeneCopoeia, Rockville, MD) were used for gene knockout transfected to 293T cells for virus packaging. PE01/PE04 and OVCAR3 cells were infected by virus 3 times and stable clones were selected by puro […]

library_books

Activation of TPA response element present in human Lemur Tyrosine Kinase 2 (lmtk2) gene increases its expression

2017
PMCID: 5645172
PMID: 29090275
DOI: 10.1016/j.bbrep.2017.09.006

[…] e element, we wanted to assess the effect of TPA on LMTK2 promoter activity. The LMTK2 promoter construct, driving the expression of a luciferase reporter gene (Gaussia luciferase), was obtained from Genecopoeia. As an expression control and for data normalization, the promoter plasmid also expressed secretory alkaline phosphatase (SEAP) under the constitutive control of a CMV promoter. This plasm […]

library_books

Molecular characterization of cell free eccDNAs in human plasma

2017
Sci Rep
PMCID: 5591271
PMID: 28887493
DOI: 10.1038/s41598-017-11368-w

[…] agen, Cat #150343) at 30 °C for 8 hours. The amplified products were purified using Agencourt AMPure XP (Beckman Coulter, A63881) and quantified using iQuant™ High Sensitivity dsDNA Quantitation Kit (GeneCopoeia, N011). […]

library_books

Silencing PRDM14 expression by an innovative RNAi therapy inhibits stemness, tumorigenicity, and metastasis of breast cancer

2017
Oncotarget
PMCID: 5564528
PMID: 28423353
DOI: 10.18632/oncotarget.16776

[…] Breast cancer cells were transfected with a HaloTag-PRDM14 lentivirus expression vector (GeneCopoeia). PRDM14-bound genomic DNA fragments were immunoprecipitated from cell lysates with HaloLink Resin (Promega, Madison, WI, USA) and immunoprecipitated with anti-H3K4me3, anti-H3K27me3, and […]

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