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GeneFisher2 | GeneFisher--software support for the detection of postulated genes

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Allows to design degenerate primers. GeneFisher2 is a web-based program that helps user to find primers for degenerated sequences. The software incorporates BatCons2 which allows to calculate ambiguous consensus sequence and Back-translation of amino-acid to nucleic-acid-sequences. The software provides four functions: Consensus Backtranslation, Codon Table Backtranslation, DNA and Primer Calculation.

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GeneFisher2 classification

GeneFisher2 specifications

Unique identifier:
OMICS_02341
Interface:
Web user interface
Input data:
Codon Usage Table, AA alignement, DNA Alignement, the consensus sequence
Output data:
The consensus sequence of the given alignment, Noise Threshold, Conservation Threshold, Cutoff Threshold, Maximal Difference of Melting Temperature, Primer Degeneracy, Display only Unique Primer, Salt Concentration, Minimal Melting Temperature, Minimal 3' GC-Content, Maximal GC-Content, Minimal Primer Length, Oligonucleotide Concentration, Minimal GC-Content, Maximal Product Size, Maximal Primer Length, Maximal Melting Temperature, Primer 3' Length, Minimal Product Size, 3' degeneracy and Nucleotide at 3'-End.
Stability:
Stable
Alternative name:
GeneFisher
Restrictions to use:
None
Input format:
CGC
Computer skills:
Basic
Maintained:
Yes

Subtools

  • BatCons2

GeneFisher2 support

Maintainers

  • Robert Giegerich <>
  • Folker Meyer <>
  • Chris Schleiermacher <>

Additional information

https://bibiserv.cebitec.uni-bielefeld.de/genefisher2?id=genefisher_manual_manual

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