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Protocols

GeneWiz specifications

Information


Unique identifier OMICS_17891
Name GeneWiz
Interface Web user interface
Restrictions to use None
Programming languages Java
Computer skills Basic
Version 0.94
Stability Stable
Maintained Yes

Maintainer


  • person_outline Peter Hallin <>

Publication for GeneWiz

GeneWiz citations

 (1620)
library_books

Optical functionalization of human Class A orphan G protein coupled receptors

2018
PMCID: 5956105
PMID: 29769519
DOI: 10.1038/s41467-018-04342-1

[…] to the intracellular elements of reference or orphan and understudied gpcrs (determined as described above) were obtained as oligonucleotides (integrated dna technologies) or synthetic genes (genewiz) and inserted into the receiver at a single or two sites in parallel. reaction mixtures (typical final volume 20 μl) contained vector (100 ng), gene fragments corresponding […]

library_books

Long noncoding RNA GAPLINC promotes gastric cancer cell proliferation by acting as a molecular sponge of miR 378 to modulate MAPK1 expression

2018
PMCID: 5957056
PMID: 29785127
DOI: 10.2147/OTT.S165147

[…] waltham, ma, usa) supplemented with 10% fbs (thermo fisher scientific) in a humidified 5% co2 atmosphere at 37°c., lncrna gaplinc sequence was purchased and subcloned into pcdna3 plasmid from genewiz (south planfield, nj, usa). mir-378 mimics or inhibitor or their respective controls were purchased from genepharma (shanghai, people’s republic of china). mapk1 sirna, gaplinc sirna, […]

library_books

Structural basis for the recognition of complex type N glycans by Endoglycosidase S

2018
PMCID: 5951799
PMID: 29760474
DOI: 10.1038/s41467-018-04300-x

[…] and endos mutants were purified as previously described with the following modifications. single-point mutations were made using the fastcloning method, and full sequences were confirmed by genewiz (https://www.genewiz.com). bl21(de3) (novagen) cells transformed with the corresponding modified form of the pcpd vector (pcpd-l) containing the c-terminal fusion protein from vibrio cholerae […]

library_books

A targeted boost and sort immunization strategy using Escherichia coli BamA identifies rare growth inhibitory antibodies

2018
PMCID: 5940829
PMID: 29740124
DOI: 10.1038/s41598-018-25609-z

[…] chain sequences of a murine anti-bama hybridoma were determined using 5′ race followed by sequencing of the pcr amplified products. cdnas encoded for both variable domains were gene synthesized (genewiz) and cloned into a mammalian surface display vector. this vector contains the light chain connect to heavy chain via an ires sequence and has a glycosylphosphatidylinositol linkage signal […]

library_books

Microbial community analysis and biodeterioration of waterlogged archaeological wood from the Nanhai No. 1 shipwreck during storage

2018
PMCID: 5940862
PMID: 29740020
DOI: 10.1038/s41598-018-25484-8

[…] 4 μl of 2.5 mm dntp mix, 2 μl of 10 μm its1 primer, 2 μl of 10 μm its4 primer, 0.5 μl of 5 u/μl transtaq-t dna polymerase (transgen biotech), and ddh2o to 50 μl. pcr products were sequenced by genewiz (beijing, china), and sequence identities were analysed using the national center for biotechnology information (ncbi) blast program (https://blast.ncbi.nlm.nih.gov/blast.cgi) and the genbank […]

library_books

Metabolic engineering of Saccharomyces cerevisiae for efficient production of glucaric acid at high titer

2018
PMCID: 5935971
PMID: 29729665
DOI: 10.1186/s12934-018-0914-y

[…] were incubated at 30 °c and 250 rpm for about 7 days., the miox4 [] gene encoding miox4 enzyme in a. thaliana and the udh gene encoding udh in p. syringae [] were codon-optimized and synthesized by genewiz (suzhou, china). to construct the py26-miox4 plasmid, the miox4 gene was first digested with bglii/noti and then ligated to the py26-gpd-tef plasmid [], which was digested with bglii/noti […]


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GeneWiz institution(s)
Center for Biological Sequence Analysis, Department of Systems Biology, The Technical University of Denmark, Lyngby, Denmark; Lersoe Parkalle 37, Copenhagen, Denmark; Roche Diagnostics Ltd., Rotkreuz, Switzerland; UC Davis Genome Center, University of California, Davis, CA, USA
GeneWiz funding source(s)
This work was supported by the Danish Center for Scientific Computing, NSF Research Grant DBI-0416764, the Danish Research Council grant 26- 06-0349, and the EU EMBRACE network of Excellence, contract number LSHG-CT-2004-512092.

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