A database containing phenotypes from RNA interference (RNAi) screens in Drosophila and human. In addition, the database provides an updated resource of RNAi reagents and their predicted quality. RNAi screening data is extracted from the literature by manual curation, but we also provide a functionality for direct submission by data producers. The new version has undergone a complete re-design of the user interface, providing an intuitive, flexible framework for additional functionalities. Screen information and gene-reagent-phenotype associations are now available for download. The integration with other resources has been improved by allowing in-links via GenomeRNAi screen IDs, or external gene or reagent identifiers. A distributed annotation system (DAS) server enables the visualization of the phenotypes and reagents in the context of a genome browser.