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geNorm specifications

Information


Unique identifier OMICS_02316
Name geNorm
Software type Package/Module
Interface Command line interface
Restrictions to use None
Operating system Unix/Linux
Computer skills Advanced
Stability Stable
Maintained No

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Publication for geNorm

geNorm citations

 (203)
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Diet Affects Muscle Quality and Growth Traits of Grass Carp (Ctenopharyngodon idellus): A Comparison Between Grass and Artificial Feed

2018
Front Physiol
PMCID: 5879129
PMID: 29632496
DOI: 10.3389/fphys.2018.00283
call_split See protocol

[…] f the target and reference genes was evaluated according to standard curves. Each experiment was conducted in triplicate. The relative stability measures (M) of the reference genes were calculated by GeNorm (http://medgen.ugent.be/genorm/). Based on the results, β-actin was chosen as the reference gene in subsequent analyses. […]

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Lactobacillus plantarum Restores Intestinal Permeability Disrupted by Salmonella Infection in Newly hatched Chicks

2018
Sci Rep
PMCID: 5797085
PMID: 29396554
DOI: 10.1038/s41598-018-20752-z
call_split See protocol

[…] to the manufacturer’s instructions. Each sample was analyzed in triplicate. Five housekeeping genes were tested after which the most stable housekeeping gene for cecal samples was selected, using the geNorm software (http://medgen.ugent.be/~jvdesomp/genorm/). GAPDH was finally chosen to normalize gene expression due to its high expression stability. The efficiency of all tested genes was between 9 […]

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Reference gene selection for qRT PCR assays in Stellera chamaejasme subjected to abiotic stresses and hormone treatments based on transcriptome datasets

2018
PeerJ
PMCID: 5888148
PMID: 29632740
DOI: 10.7717/peerj.4535
call_split See protocol

[…] Three different types of statistical tools: geNorm (version 3.5), NormFinder (version 0.953), and BestKeeper (version 1.0), were applied to rank the expression stability of the RGs across all of the experimental sets. For geNorm and NormFinder, […]

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CRTC1 mediates preferential transcription at neuronal activity regulated CRE/TATA promoters

2017
Sci Rep
PMCID: 5740062
PMID: 29269871
DOI: 10.1038/s41598-017-18215-y
call_split See protocol

[…] or cultured neurons or the geometric mean of Gapdh, hypoxanthine guanine phosphoribosyl transferase (Hprt) and peptidylprolyl isomerase A (Ppia) for brain samples using the NormFinder, BestKeeper and geNorm algorithms. […]

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Association of innate defense proteins BPIFA1 and BPIFB1 with disease severity in COPD

2017
PMCID: 5741069
PMID: 29296079
DOI: 10.2147/COPD.S144136
call_split See protocol

[…] 95°C and 15 s at 60°C). Data were analyzed using the standard curve method, and the expressions of BPIFA1 and BPIFB1 were calculated relative to the expression of the three reference genes, using the geNorm applet (http://medgen.ugent.be/~jvdesomp/genorm/). […]

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Reference gene validation for gene expression normalization in canine osteosarcoma: a geNorm algorithm approach

2017
PMCID: 5702123
PMID: 29178874
DOI: 10.1186/s12917-017-1281-3

[…] The average reference gene expression stability (M value) upon step-wise exclusion and pairwise variation (V value) were calculated using the geNorm algorithm approach for the tumors and cell lines individually. A higher absolute M value indicates lower expression stability and vice versa (Table ). Among the reference genes tested for the c […]

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geNorm institution(s)
Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Utrecht University, Utrecht, Netherlands

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