GETPrime statistics

info info

Citations per year

info

Tool usage distribution map

info info

Associated diseases

info

Popular tool citations

chevron_left Primer databases chevron_right
Want to access the full stats & trends on this tool?

Protocols

GETPrime specifications

Information


Unique identifier OMICS_18618
Name GETPrime
Restrictions to use None
Database management system MySQL
Community driven No
Data access File download, Browse, Application programming interface
User data submission Not allowed
Maintained Yes

Taxon


  • Invertebrates
    • Caenorhabditis elegans
    • Drosophila melanogaster
  • Plants and Fungi
    • Saccharomyces cerevisiae
  • Primates
    • Homo sapiens
  • Rodents
    • Mus musculus
    • Rattus norvegicus
  • Vertebrates
    • Danio rerio
    • Gallus gallus

Maintainers


  • person_outline Jacques Rougemont
  • person_outline Bart Deplancke

Publications for GETPrime

GETPrime citations

 (6)
library_books

X Chromosome Crossover Formation and Genome Stability in Caenorhabditis elegans Are Independently Regulated by xnd 1

2016
PMCID: 5144962
PMID: 27678523
DOI: 10.1534/g3.116.035725

[…] al-time PCRs were performed on the Applied Bio Systems 7300 Real Time PCR System using SYBR Green chemistry (SensiMix SYBR Hi-ROX kit, Bioline #QT-605) with transcript-specific primers designed using GETPrime and Primer3 (Table S2; ; ; ). The reference genes rpl-32 and Y45F10D.4 () were used for normalization across samples and gene expression was analyzed using the ΔCT method (). Results are pres […]

library_books

Longevity Genes Revealed by Integrative Analysis of Isoform Specific daf 16/FoxO Mutants of Caenorhabditis elegans

2015
Genetics
PMCID: 4596673
PMID: 26219299
DOI: 10.1534/genetics.115.177998

[…] and a Mastercycler ep realplex thermal cycler (Eppendorf North America, Westbury, NY). A total of 10 ng of cDNA was used as a template in 15 µl reaction volume. Primers were selected initially using GETPrime () and Primer-BLAST (http://www.ncbi.nlm.nih.gov/tools/primer-blast/) and subsequently validated by melt curve analysis and agarose gel electrophoresis. Primer sequences are listed in Table S […]

call_split

GABA A and NMDA receptor subunit mRNA expression is altered in the caudate but not the putamen of the postmortem brains of alcoholics

2014
PMCID: 4257153
PMID: 25538565
DOI: 10.3389/fncel.2014.00415
call_split See protocol

[…] 5 × SYBR Green I (Invitrogen), and 0.4 μM each of forward and reverse primers. The gene-specific primer pairs (primer sequences shown in Table in Supplementary Material) were designed using database GETPrime, EPFL (Gubelmann et al., ) or Primer Express Software version 3.0 (Applied Biosystems) and then validated using hippocampal cDNA from human brain by identification of a single peak in the mel […]

call_split

Expression of specific ionotropic glutamate and GABA A receptor subunits is decreased in central amygdala of alcoholics

2014
PMCID: 4165314
PMID: 25278838
DOI: 10.3389/fncel.2014.00288
call_split See protocol

[…] transcriptase in the reaction was omitted which served as a negative control. The gene-specific primer pairs (primer sequences shown in Supplementary Table ) were designed using NCBI Primer-BLAST and GETPrime (updepla1srv1.epfl.ch/getprime/) and validated with BioBank cDNAs from human brains (PrimerDesign Ltd., UK) by the identification of a single peak in the melting curve and a single band with […]

call_split

Selective increases of AMPA, NMDA, and kainate receptor subunit mRNAs in the hippocampus and orbitofrontal cortex but not in prefrontal cortex of human alcoholics

2014
PMCID: 3905203
PMID: 24523671
DOI: 10.3389/fncel.2014.00011
call_split See protocol

[…] × SYBR Green I (Invitrogen) and 0.4 μ M each of forward and reverse primers. The gene-specific primer pairs (primer sequences shown in supplementary Table ) were designed using NCBI Primer-blast and GETPrime (updepla1srv1.epfl.ch/getprime/) synthesized by SigmaAldrich and further validated with cDNA from human brain. Amplification was performed in 384-well optical plates using the ABI PRISM 7900H […]

call_split

Global and Stage Specific Patterns of Krüppel Associated Box Zinc Finger Protein Gene Expression in Murine Early Embryonic Cells

2013
PLoS One
PMCID: 3579818
PMID: 23451074
DOI: 10.1371/journal.pone.0056721
call_split See protocol

[…] n on a 7900HT Fast Real-Time PCR machine (Applied Biosystem) using SybrGreen Master Mix (Roche). Primers were designed either with the Primer Express software (Applied Biosystem) or with the resource GETPrime (http://updepla1srv1.epfl.ch/getprime ). For a complete list of primers, see . Primer specificity was confirmed by dissociation curve analysis. Normalization of qPCR data was performed on val […]


Want to access the full list of citations?
GETPrime institution(s)
Bioinformatics and Biostatistics Core Facility, School of Life Sciences, Ecole Polytechnique Fedérale de Lausanne (EPFL), Lausanne, Switzerland; Swiss Institute of Bioinformatics, Lausanne, Switzerland; Laboratory of Systems Biology and Genetics, Institute of Bio-engineering, School of Life Sciences, Ecole Polytechnique Fedérale de Lausanne (EPFL), Lausanne, Switzerland
GETPrime funding source(s)
Supported by Swiss National Science Foundation Grant [#31003A 16273 5]; SyBIT project of SystemsX.ch; Swiss Federal Institute of Technology in Lausanne (EPFL).

GETPrime reviews

star_border star_border star_border star_border star_border
star star star star star

Be the first to review GETPrime