GPI

[…] tional file . They were designed based on Gene Bank data http://www.ncbi.nlm.nih.gov/Genbank/ for aquaporins using the accession numbers provided in [] and based on the maize EST assembly produced by Genoplante-Info and now housed at the URGI http://urgi.versailles.inra.fr/GnpSeq/ for LEA, RAB and other maturation-related genes.For qRT-PCR, RNA isolation was carried out essentially as previously d […]

[…] ctions of the subcellular localizations of various known mitochondrion and plastid proteins and the tomato GABA-T isoforms were obtained using the web-based bioinformatic programs Predotar () (http://genoplante-info.infobiogen.fr/predotar/), TargetP () (http://www.cbs.dtu.dk/services/TargetP/), MitoProt () (http://ihg2.helmholtz-muenchen.de/ihg/mitoprot.html), ChloroP () (http://www.cbs.dtu.dk/ser […]

[…] rimary and secondary cell wall biosynthesis and assembly for all species throughout the plant kingdom. Maize orthologous genes were found from BLAST (blastn and tblastx) searches [] performed against GenoPlante-Info databases [].Membrane hybridizations and data analyses were achieved according to a protocol adapted from Pesquet et al. [] and developed in Guillaumie et al. []. Each GST was diluted […]

[…] ww.expasy.org/tools/) predicted the existence of a transit peptide that varied in length from 29 to 36 amino acids (), although the subcellular location of GR2 was ambiguous, with Predator () (http://genoplante-info.infobiogen.fr/predotar/), and TargetP favouring a mitochondrial location, and ChloroP and PSORT favouring a plastidial location (data not shown). As a compromise, in this study the N-t […]

[…] MTS2 from the hypocotyl and root cDNA library. Sequences can be found in Genbank as AY840091 and AY840092, respectively. The presence of plastid targeting signals was predicted using PREDOTAR (http://genoplante-info.infobiogen.fr/predotar/predotar.html; Small et al. ) and TargetP (http://www.cbs.dtu.dk/services/TargetP/; Emanuelsson et al. ). […]

[…] (http://www.ncbi.nlm.nih.gov), and MIPS (http://mips.gsf.de/). Subcellular localization was predicted using either TargetP V1.0 [] (http://www.cbs.dtu.dk/services/TargetP/) or PredoTar V1.03 (http://genoplante-info.infobiogen.fr/predotar/predotar.html). The deduced amino acid sequences were aligned using the CLUSTALX program [] with the default parameter settings and manually improved with respec […]