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GPQuest specifications


Unique identifier OMICS_19832
Name GPQuest
Software type Application/Script
Interface Command line interface
Restrictions to use None
Operating system Unix/Linux
Computer skills Advanced
Stability Stable
Maintained Yes


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  • person_outline Hui Zhang <>

Publication for GPQuest

GPQuest in publications

PMCID: 5800646
PMID: 29370305
DOI: 10.1371/journal.ppat.1006812

[…] (thermo scientific). hiv-1 peptides were identified by using sequest in proteome discoverer software (thermo fisher scientific, version 2.2). the intact env glycopeptides were identified using gpquest [, ]. to quantify the glycosylation in different mutants label-free quantification of env peptides and glycopeptides was done using thermo sieve software version 2.1 (). the data […]

PMCID: 5585273
PMID: 28874712
DOI: 10.1038/s41467-017-00535-2

[…] comprehensive glycopeptide fragments than the conventional hcd-ms/ms spectra reported in ngag (supplementary fig. ). our search engine pglyco 2.0 showed better performance than the search engine gpquest used in ngag: pglyco 2.0 identified 97% more glycopeptide spectra than gpquest in the analysis of the same ms/ms data from ngag under the same peptide fdr (supplementary fig.  and methods). […]

PMCID: 5116676
PMID: 27869200
DOI: 10.1038/srep37189

[…] assignment of glycopeptides to tandem spectra and reduces the size of search space, thus improving the performance of the data mining process. this classification tactic has been incorporated into gpquest. together with a refined decoy and scoring algorithm, gpquest has been further improved to identify site-specific n- and o-glycosylation in the hcd ms/ms spectra of intact glycopeptides., […]

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GPQuest institution(s)
Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, USA; Department of Pathology, School of Medicine, Johns Hopkins University, Baltimore, MD, USA
GPQuest funding source(s)
Supported by the National Institutes of Health under grants and contracts of National Cancer Institute, Clinical Proteomics Tumor Analysis Consortium (U24CA160036), the Early Detection Research Network (EDRN, U01CA152813 and U24CA115102), and R01CA112314; National Heart Lung Blood Institute, Program of Excellence in Glycosciences (P01HL107153), NHLBI Proteomic Center (N01-HV-00240).

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