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GPQuest specifications


Unique identifier OMICS_19832
Name GPQuest
Software type Application/Script
Interface Command line interface
Restrictions to use None
Operating system Unix/Linux
Computer skills Advanced
Stability Stable
Maintained Yes


No version available


  • person_outline Hui Zhang

Publication for GPQuest

GPQuest citations


Alterations of HIV 1 envelope phenotype and antibody mediated neutralization by signal peptide mutations

PLoS Pathog
PMCID: 5800646
PMID: 29370305
DOI: 10.1371/journal.ppat.1006812

[…] ometer (Thermo Scientific). HIV-1 peptides were identified by using SEQUEST in Proteome Discoverer software (Thermo Fisher Scientific, version 2.2). The intact Env glycopeptides were identified using GPQuest [, ]. To quantify the glycosylation in different mutants label-free quantification of Env peptides and glycopeptides was done using Thermo SIEVE software version 2.1 (). The data was normalize […]


Classification of Tandem Mass Spectra for Identification of N and O linked Glycopeptides

Sci Rep
PMCID: 5116676
PMID: 27869200
DOI: 10.1038/srep37189

[…] of fetuin, ovalbumin and glycophorin A were converted to mzXML files using the Trans-Proteomic Pipeline (TPP), with the “centroid all scans” option selected. The precursor mass matching algorithm on GPQuest was used to analyse the mzXML files. The following parameters were used in GPQuest: Mass tolerance was set to 10 and 20 ppm at MS1 and MS2 levels, respectively. N- and O-glycosylation analysis […]

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GPQuest institution(s)
Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, USA; Department of Pathology, School of Medicine, Johns Hopkins University, Baltimore, MD, USA
GPQuest funding source(s)
Supported by the National Institutes of Health under grants and contracts of National Cancer Institute, Clinical Proteomics Tumor Analysis Consortium (U24CA160036), the Early Detection Research Network (EDRN, U01CA152813 and U24CA115102), and R01CA112314; National Heart Lung Blood Institute, Program of Excellence in Glycosciences (P01HL107153), NHLBI Proteomic Center (N01-HV-00240).

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