GRAVY Calculator statistics

Tool stats & trends

Looking to identify usage trends or leading experts?


GRAVY Calculator specifications


Unique identifier OMICS_13605
Name GRAVY Calculator
Interface Web user interface
Restrictions to use None
Input data Protein sequence(s)
Input format FASTA
Output data Results could be clearly arranged in single sections; Results could be arranged in a table. This is recommended for multi-sequence queries; Separator-delimited result view for data export.
Computer skills Basic
Stability Stable
Maintained Yes


  • person_outline GRAVY Calculator Team

GRAVY Calculator citations


Fast and Simple Protocols for Mass Spectrometry Based Proteomics of Small Fresh Frozen Uterine Tissue Sections

Anal Chem
PMCID: 5647562
PMID: 28910098
DOI: 10.1021/acs.analchem.7b01937
call_split See protocol

[…] d using normalized spectral abundance factor (NSAF) reported by Peptideshaker. Protein sequences were obtained from the UniprotKB Web site (, and GRAVY indexes were obtained using the GRAVY calculator ( […]


Comparison of fractionation proteomics for local SWATH library building

PMCID: 5601298
PMID: 28664598
DOI: 10.1002/pmic.201700052

[…] GRAVY scores were determined using, and pI and molecular weight using The SWATH Acquisition Replicates Template was used to evaluate the reproducibility of the transitions based o […]


Comparative Lipidomics and Proteomics of Lipid Droplets in the Mesocarp and Seed Tissues of Chinese Tallow (Triadica sebifera)

Front Plant Sci
PMCID: 5541829
PMID: 28824675
DOI: 10.3389/fpls.2017.01339
call_split See protocol

[…] algorithm, using the ProtScale program at The value G in each graph is the grand average of hydropathy (GRAVY) for each protein and was calculated using the GRAVY calculator program at alignments of oleosin amino acid sequence were performed using the ClustalW algorithm of Geneious 9.0 (Kearse et al., ) an […]


Identifying components required for OMP biogenesis as novel targets for antiinfective drugs

PMCID: 5711350
PMID: 28118090
DOI: 10.1080/21505594.2016.1278333
call_split See protocol

[…] p:// Username: [email protected] Password: XBxYXb4E) and in Table S4. Hydropathy indices of proteins were calculated using the tool […]


In silico functional elucidation of uncharacterized proteins of Chlamydia abortus strain LLG

PMCID: 5351547
PMID: 28344832
DOI: 10.4155/fsoa-2016-0066

[…] is regarded as the positive factor for stability at high temperature. For better interaction of proteins with water molecules, very low GRAVY index is considered as helpful and was calculated through GRAVY calculator by the total sum of values of hydropathy for all amino acids, divided by the total length of the protein. Aliphatic and aromatic properties with average number of polar and nonpolar a […]


Shotgun proteomics of the barley seed proteome

BMC Genomics
PMCID: 5219712
PMID: 28061743
DOI: 10.1186/s12864-016-3408-5

[…] Protein sequences of the barley seed proteome in FASTA format were obtained from the Uniprot database. The grand average of hydropathic value (GRAVY) was calculated using the gravy calculator ( The hydropathy plot was generated using MS Excel. […]


Looking to check out a full list of citations?

GRAVY Calculator reviews

star_border star_border star_border star_border star_border
star star star star star

Be the first to review GRAVY Calculator