GRAVY Calculator specifications


Unique identifier OMICS_13605
Name GRAVY Calculator
Interface Web user interface
Restrictions to use None
Input data Protein sequence(s)
Input format FASTA
Output data Results could be clearly arranged in single sections; Results could be arranged in a table. This is recommended for multi-sequence queries; Separator-delimited result view for data export.
Computer skills Basic
Stability Stable
Maintained Yes


  • person_outline GRAVY Calculator Team <>

GRAVY Calculator in publications

PMCID: 5852485
PMID: 29439417
DOI: 10.3390/md16020057

[…] for the improvement of the manuscript. , the authors declare that there is no conflict of interests., nn: no data; a pi and gravy were calculated by and; b data not published in our work., nn: no data; a mic: μg/ml; trinary tract infection (uti); b ic50: μm; c zebrafish model; d ic50: μg/ml; e embryos challenged with aeromonas […]

PMCID: 5647562
PMID: 28910098
DOI: 10.1021/acs.analchem.7b01937

[…] using normalized spectral abundance factor (nsaf) reported by peptideshaker. protein sequences were obtained from the uniprotkb web site (, and gravy indexes were obtained using the gravy calculator (, the peptide mixtures obtained from proteolysis of protein extracts of tissues are complex samples, and interaction between different compounds […]

PMCID: 5601298
PMID: 28664598
DOI: 10.1002/pmic.201700052

[…] peptides were not imported. all information (including areas, score, fdr, observed retention time) was exported in .xlsx format for results analysis., gravy scores were determined using, and pi and molecular weight using the swath acquisition replicates template was used to evaluate the reproducibility of the transitions based […]

PMCID: 5541829
PMID: 28824675
DOI: 10.3389/fpls.2017.01339

[…] using the protscale program at the value g in each graph is the grand average of hydropathy (gravy) for each protein and was calculated using the gravy calculator program at, multiple-sequence alignments of oleosin amino acid sequence were performed using the clustalw algorithm of geneious 9.0 (kearse et al., ) […]

PMCID: 5539426
PMID: 28765221
DOI: 10.1128/mBio.00909-17

[…] for ompa and dsba, the h-region for each variant was defined using phobius () ( hydrophobicity was scored using the grand average of hydropathy (gravy) value at wt, wild type. bold letter k in sequences indicates inserted lysine residue., when we examined growth on sds semiquantitatively by monitoring growth curves (), it could be seen […]

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