Green and Red Puncta Colocalization statistics

Tool stats & trends

Looking to identify usage trends or leading experts?

Green and Red Puncta Colocalization specifications

Information


Unique identifier OMICS_25495
Name Green and Red Puncta Colocalization
Software type Package/Module
Interface Graphical user interface
Restrictions to use None
Operating system Unix/Linux, Mac OS, Windows
Programming languages Java
License GNU General Public License version 3.0
Computer skills Medium
Stability Stable
Requirements
ImageJ
Source code URL http://imagejdocu.tudor.lu/lib/exe/fetch.php?media=plugin:analysis:colocalization_analysis_macro_for_red_and_green_puncta:red_and_green_puncta_image_j_website._enhancedtxt.txt
Maintained Yes

Download


download.png

Versioning


No version available

Maintainer


  • person_outline Ruben Dagda

Green and Red Puncta Colocalization citations

 (9)
library_books

CD5L Promotes M2 Macrophage Polarization through Autophagy Mediated Upregulation of ID3

2018
Front Immunol
PMCID: 5858086
PMID: 29593730
DOI: 10.3389/fimmu.2018.00480

[…] nder an Axio Observer Z1 DUO LSM 710 confocal system and analyzed with ZEN Black software (Carl Zeiss Microscopy GmbH). LC3 and LC3-LysoTracker Red colocalized puncta per cell were determined using a green and red puncta colocalization macro and ImageJ software in threshold images with sizes from 3 to 30 pixel2 and puncta circularity 0.8–1, as described previously (). […]

library_books

Stall in Canonical Autophagy Lysosome Pathways Prompts Nucleophagy Based Nuclear Breakdown in Neurodegeneration

2017
Curr Biol
PMCID: 5723708
PMID: 29174892
DOI: 10.1016/j.cub.2017.10.054

[…] , ‘analyze particles’ command on binary images was applied. For intensity values gray level images were assessed after background subtraction. Colocalization analysis performed using images from the ‘Green and Red Puncta Colocalization’ macro []. Lipofuscin quantification was performed manually using the cell counter tool in ImageJ. The automated quantification of LaminB1 inclusions in the cytopla […]

library_books

GABARAPL1 tumor suppressive function is independent of its conjugation to autophagosomes in MCF 7 breast cancer cells

2017
Oncotarget
PMCID: 5593540
PMID: 28915569
DOI: 10.18632/oncotarget.19639

[…] et (Vector laboratories, H1400). The cells were then examined and photographed using a confocal microscope (Olympus Fluoview FV1000). For ptf-LC3, green, red and yellow puncta were counted using the “Green and Red puncta colocalization tool” designed for ImageJ. For each experiment, 20 cells were randomly selected.For Lysotracker staining, cells duplicate were stained with 500 nM of LysoTracker® R […]

library_books

Interferon inducible protein SCOTIN interferes with HCV replication through the autolysosomal degradation of NS5A

2016
Nat Commun
PMCID: 4754343
PMID: 26868272
DOI: 10.1038/ncomms10631

[…] riton X-100 (TBST) instead of PBS or PBST. Pearson's correlation coefficient was calculated using the JACoP plugin for Image J. Quantification of RFP+/GFP+ or RFP+/GFP− puncta was performed using the Green and Red Puncta Colocalization Macro for Image J (Daniel J. Shiwarski, Ruben K. Dagda and Charleen T. Chu). […]

library_books

The human CD5L/AIM CD36 axis: A novel autophagy inducer in macrophages that modulates inflammatory responses

2015
Autophagy
PMCID: 4502645
PMID: 25713983
DOI: 10.1080/15548627.2015.1017183

[…] onal Institutes of Health). RELA translocation was quantified in 250 cells from 3 independent experiments as described. LC3 and LC3-LysoTracker Red colocalized puncta per cell were determined using a green and red puncta colocalization macro and ImageJ software in threshold images with sizes from 3 to 30 pixel2 and puncta circularity 0.8 to 1 in more than 50 cells from 3 independent experiments sc […]

library_books

Connexins modulate autophagosome biogenesis

2014
Nat Cell Biol
PMCID: 4008708
PMID: 24705551
DOI: 10.1038/ncb2934

[…] staining with antibodies against endogenous LC3. Autophagic flux was also tested by transient transfection of the mCherry-GFP-LC3 plasmid. Quantification of the yellow and red puncta was performed by Green and Red Puncta Colocalization Macro for ImageJ (D. J. Swiwarski modified by R.K. Dagda). […]

Citations

Looking to check out a full list of citations?

Green and Red Puncta Colocalization reviews

star_border star_border star_border star_border star_border
star star star star star

Be the first to review Green and Red Puncta Colocalization