Designs custom Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) screens targeting sets of genes defined by the user. Green Listed is a web-based tool facilitating the rapid design of custom CRISPR screens. It is used to mine publicly available source lists/reference libraries of gRNAs to design custom libraries targeting genes specified by the user, such as all kinases or all genes up-regulated in an RNAseq experiment. Green Listed enables researchers to rapidly design custom CRISPR screens against a selected list of genes.
Department of Medicine, Solna, Karolinska Institutet, Karolinska University Hospital, Stockholm, Sweden; Department of Marine Sciences, Lundberg Laboratory, University of Gothenburg, Gothenburg, Sweden
Green Listed funding source(s)
This project was financially supported by the Swedish Research Council, the Swedish Foundation for Strategic Research, the Wenner-Gren Foundations, the Jeansson Foundations, the Karolinska Institutet and BILS (National Bioinformatics Infrastructure for Life Sciences).