Provides a universal CRISPR annotation system. grID is an extensive compilation of gRNA properties including sequence and variations, thermodynamic parameters, off-target analyses, and alternative PAM sites, among others. The database is designed to keep up with the rapidly evolving CRISPR technology. Users can search in the database by NCBI reference sequence ID, Gene Symbol or any valid 23-bp targeting sequence in the form N20NGG.

User report

0 user reviews

0 user reviews

No review has been posted.

grID forum

No open topic.

grID classification

grID specifications

Restrictions to use:
None
Database management system:
MySQL
Data access:
Web user interface
Programming languages:
Javascript
Community driven:
No
User data submission:
Not allowed

Credits

Publications

  • grID: A CRISPR-Cas9 guide RNA Database and Resource for Genome-Editing.
    Published in: 2016
    DOI: 10.1101/097352

Institution(s)

Department of Ophthalmology, Wilmer Eye Institute, The Johns Hopkins University School of Medicine, USA; Department of Molecular Biology and Genetics, The Johns Hopkins University School of Medicine, USA; Solomon H. Snyder Department of Neuroscience, The Johns Hopkins University School of Medicine, USA; Institute of Genetic Medicine, The Johns Hopkins University School of Medicine, USA

Funding source(s)

This work was supported by a Knights Templar Career Starter Research Grant, grants from the National Institutes of Health (P30EY001765, EY009769), unrestricted funds from Research to Prevent Blindness, Inc., funds from Foundation Fighting Blindness, and generous gifts from the Guerrieri Family Foundation and by funded MRI-R2 project #DBI-0959894.

By using OMICtools you acknowledge that you have read and accepted the terms of the end user license agreement.