Provides a universal CRISPR annotation system. grID is an extensive compilation of gRNA properties including sequence and variations, thermodynamic parameters, off-target analyses, and alternative PAM sites, among others. The database is designed to keep up with the rapidly evolving CRISPR technology. Users can search in the database by NCBI reference sequence ID, Gene Symbol or any valid 23-bp targeting sequence in the form N20NGG.

User report

0 user reviews

0 user reviews

No review has been posted.

grID forum

No open topic.

grID classification

grID specifications

Restrictions to use:
None
Database management system:
MySQL
Data access:
Web user interface
Programming languages:
Javascript
Community driven:
No
User data submission:
Not allowed

Publications

  • (Jaskula-Ranga and Zack, 2016) grID: A CRISPR-Cas9 guide RNA Database and Resource for Genome-Editing. bioRxiv.
    DOI: 10.1101/097352

Credits

Institution(s)

Department of Ophthalmology, Wilmer Eye Institute, The Johns Hopkins University School of Medicine, USA; Department of Molecular Biology and Genetics, The Johns Hopkins University School of Medicine, USA; Solomon H. Snyder Department of Neuroscience, The Johns Hopkins University School of Medicine, USA; Institute of Genetic Medicine, The Johns Hopkins University School of Medicine, USA

Funding source(s)

This work was supported by a Knights Templar Career Starter Research Grant, grants from the National Institutes of Health (P30EY001765, EY009769), unrestricted funds from Research to Prevent Blindness, Inc., funds from Foundation Fighting Blindness, and generous gifts from the Guerrieri Family Foundation and by funded MRI-R2 project #DBI-0959894.

Related CRISPR/Cas9 screen analysis tools

Most Recent Tools

Web app
G T A T C G C T A CRISPR-enabled… CRISPR-enabled trackable genome engineering

CREATE CRISPR-enabled trackable genome engineering

Links each guide RNA to homologous repair cassettes that both edit loci and…

Links each guide RNA to homologous repair cassettes that both edit loci and function as barcodes to track genotype-phenotype relationships. CREATE combines automated design of CREATE cassettes…

Desktop app
G T A T C G C T A Model-based Analysis… Model-based Analysis of Genome-wide CRISPR/Cas9…

MAGeCK Model-based Analysis of Genome-wide CRISPR/Cas9 Knockout

Identifies positively and negatively selected sgRNAs and genes in genome-scale…

Identifies positively and negatively selected sgRNAs and genes in genome-scale CRISPR/Cas9 knockout experiments. It consists of four steps: read count normalization, mean-variance modeling, sgRNA…

Desktop app
G T A T C G C T A CRISPR-AnalyzeR for… CRISPR-AnalyzeR for pooled screens

caRpools CRISPR-AnalyzeR for pooled screens

An R package for exploratory data analysis that provides a complete workflow to…

An R package for exploratory data analysis that provides a complete workflow to analyze CRISPR/Cas9 screens. To further support the analysis of large-scale screens, caRpools integrates screening…

Desktop app
G T A T C G C T A Screening Bayesian… Screening Bayesian Evaluation and Analysis Method

ScreenBEAM Screening Bayesian Evaluation and Analysis Method

A hierarchical modelling algorithm within Bayesian framework for meta-analysis…

A hierarchical modelling algorithm within Bayesian framework for meta-analysis of large-scale FG screens. This novel multi-probe approach performs more robustly than previously established analysis…

1 related tool

By using OMICtools you acknowledge that you have read and accepted the terms of the end user license agreement.