HDI Software statistics

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Citations per year

Number of citations per year for the bioinformatics software tool HDI Software

Tool usage distribution map

This map represents all the scientific publications referring to HDI Software per scientific context
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Associated diseases


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HDI Software specifications


Unique identifier OMICS_29457
Name HDI Software
Alternative name High Definition Imaging Software
Software type Application/Script
Interface Graphical user interface
Restrictions to use License purchase required
Operating system Unix/Linux
Computer skills Medium
Stability Stable
Registration required Yes
Maintained Yes


No version available


  • person_outline Team Waters

Additional information

http://www.waters.com/waters/library.htm?locale=en_US&cid=134833914&lid=134913584 http://www.waters.com/waters/library.htm?locale=en_US&cid=134833914&lid=134886225

HDI Software citations


MALDI Mass Spectrometry Imaging: A Novel Tool for the Identification and Classification of Amyloidosis

PMCID: 5725723
PMID: 28994248
DOI: 10.1002/pmic.201700236

[…] IMS parameters trap bias direct current (DC), wave velocity (WV), and transfer wave velocity (TWV) were set at 70 V, 350 m/s, and 175 m/s, respectively. Regions for acquisitions were defined with the High Definition Imaging software (HDI) (v1.3.5, Waters) on imported digital scans (Epson Perfection 1640SU) of the tissue sections. Peptide images were generated with the Apex3D algorithm (m/z window: […]


Panax ginseng genome examination for ginsenoside biosynthesis

PMCID: 5710592
PMID: 29048480
DOI: 10.1093/gigascience/gix093

[…] the sample to produce the MS spectra from the surface, which was then rastered across the sample at regular intervals to build a 2D image. Image creation was performed using high-definition imaging (HDI) software (Waters Corporation) with the following parameters: X and Y pixel size 100 μm; raster speed 400 μm/s; spray solvent 90% MeOH, 10% H2O, 0.1 mM NH4Cl, and 0.1 mM leucine enkephalin deliver […]


Mass spectrometric monitoring of interfacial photoelectron transfer and imaging of active crystalline facets of semiconductors

Nat Commun
PMCID: 5322523
PMID: 28224986
DOI: 10.1038/ncomms14524

[…] s. The step size has been set as 80 × 80 μm. For each pixel, the acquisition time is 1 s. Potential differences between the sample plate and the aperture were set as 20 V for all imaging experiments. HDI software (Waters, US) was used for imaging analysis. […]


Rapid Detection of Necrosis in Breast Cancer with Desorption Electrospray Ionization Mass Spectrometry

Sci Rep
PMCID: 5062153
PMID: 27734938
DOI: 10.1038/srep35374

[…] Average spectra from two distinct Regions of Interest (ROI) from viable/necrotic tissue (guided by pathology) from each tumor were defined through the HDI software, from which a list of ions was extracted (i.e. data point). We used m/z values to two decimal points. Using the MetaboAnalyst platform (http://www.metaboanalyst.ca), with a mass tolerance […]


Lipid changes within the epidermis of living skin equivalents observed across a time course by MALDI MS imaging and profiling

PMCID: 4525729
PMID: 26243140
DOI: 10.1186/s12944-015-0089-z

[…] 00 in the positive mode. The acquisition of data was performed at a spatial resolution of 50 μm × 50 μm, with the laser energy set to 185 arbitrary units. The data was then processed using the Waters HDI software (Waters Corporation, UK). The average spectra along with the average of ion molecule drift time units were observed simultaneously with spatial abundance of ions within the images. The im […]


Alternative Surfactants for Improved Efficiency of In Situ Tryptic Proteolysis of Fingermarks

J Am Soc Mass Spectrom
PMCID: 4422860
PMID: 25916599
DOI: 10.1007/s13361-015-1140-z

[…] orm mass spectrometry software, or processed directly within MassLynx by means of peak smoothing, baseline correction, and peak centroiding. MSI data were processed using the high definition imaging (HDI) software (Waters Corporation). Expasy (http://www.expasy.org/) was employed to generate in silico peptide lists of known proteins present in fingermarks (from examining the literature available) […]

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HDI Software institution(s)
Waters Corporation, Milford, MA, USA

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