HiFive specifications

Information


Unique identifier OMICS_08417
Name HiFive
Software type Toolkit/Suite
Interface Command line interface
Restrictions to use None
Operating system Unix/Linux
Programming languages Python
Parallelization MPI
Computer skills Advanced
Stability Stable
Requirements
Scipy, Numpy, Cython, h5py
Maintained Yes

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Documentation


Maintainer


  • person_outline Michael E.G. Sauria <>

Information


Unique identifier OMICS_08417
Name HiFive
Interface Web user interface
Restrictions to use None
Programming languages Python
Computer skills Basic
Stability Stable
Maintained Yes

Documentation


Maintainer


  • person_outline Michael E.G. Sauria <>

Publication for HiFive

HiFive in publications

 (12)
PMCID: 5940772
PMID: 29739942
DOI: 10.1038/s41467-018-04214-8

[…] gene (geneart, regensburg, germany) into pfastbachtb vector for expression in the baculovirus system following standard procedures (bac-to-bac manual, invitrogen). the protein was expressed in hifive insect cells in express five sfm medium (thermofisher scientific, b85502) supplemented with glutamine according to standard protocols. for purification, a cell pellet was resuspended in buffer […]

PMCID: 5346121
PMID: 28078515
DOI: 10.1007/s10577-016-9546-4

[…] will naturally occur more often than other contacts of the same distance. there are a number of methods that apply this idea for hi-c, e.g. fit-hi-c (ay et al. ), homer (heinz et al. ) and hifive (sauria et al. ). for capture hi-c, so far, there is only chicago (cairns et al. ) that uses distance correction, taking into account the different properties of bait-bait and bait-non-bait […]

PMCID: 4990487
PMID: 27428511
DOI: 10.1038/nchembio.2128

[…] the g. dicer and rrm*-dicer were inserted into a modified pfastbac1 vector (invitrogen) which has an n-terminal glutathione-s-transferase (gst) tag. the gst fusion proteins were expressed in hifive insect cells following standard procedures and isolated by glutathione affinity chromatography (ge healthcare: gstrap ff) using buffer containing 1×pbs and 5 mm dtt. after on-column cleavage […]

PMCID: 4016208
PMID: 24816721
DOI: 10.1371/journal.pone.0097030

[…] of the polyhedrin promoter of pfastbac dual plasmid (invitrogen, fisherscientific, illkirch, france) and then used to generate recombinant baculoviruses using the bac-to-bac system (invitrogen). hifive cells maintained in grace medium (invitrogen) were infected with the different recombinant baculoviruses for production of the polyomavirus vlps. vlps were then purified by ultracentrifugation […]

PMCID: 3622673
PMID: 23593196
DOI: 10.1371/journal.pone.0060334

[…] total number of cells counted per sample was 10,000., the interaction of selected compounds with abcb1 was assessed by an in vitro photolabeling assay as previously described . crude membranes from hifive cells overexpressing human abcb1 were incubated with the selected compounds for 5 minutes, after which 3 to 5 nmol/l [125i]iaap in 50 mm tris-hcl (ph 7.5) was added. after exposure to uv light […]


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HiFive institution(s)
Departments of Biology and Computer Science, Johns Hopkins University, Baltimore, MD, USA

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