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HiFive specifications

Information


Unique identifier OMICS_08417
Name HiFive
Software type Toolkit/Suite
Interface Command line interface
Restrictions to use None
Operating system Unix/Linux
Programming languages Python
Parallelization MPI
Computer skills Advanced
Stability Stable
Requirements
Scipy, Numpy, Cython, h5py
Maintained Yes

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Maintainer


  • person_outline Michael E.G. Sauria

Information


Unique identifier OMICS_08417
Name HiFive
Interface Web user interface
Restrictions to use None
Programming languages Python
Computer skills Basic
Stability Stable
Maintained Yes

Documentation


Maintainer


  • person_outline Michael E.G. Sauria

Publication for HiFive

HiFive citations

 (12)
library_books

Molecular mechanism of influenza A NS1 mediated TRIM25 recognition and inhibition

2018
Nat Commun
PMCID: 5940772
PMID: 29739942
DOI: 10.1038/s41467-018-04214-8

[…] gene (geneart, regensburg, germany) into pfastbachtb vector for expression in the baculovirus system following standard procedures (bac-to-bac manual, invitrogen). the protein was expressed in hifive insect cells in express five sfm medium (thermofisher scientific, b85502) supplemented with glutamine according to standard protocols. for purification, a cell pellet was resuspended in buffer […]

library_books

Capturing genomic relationships that matter

2017
PMCID: 5346121
PMID: 28078515
DOI: 10.1007/s10577-016-9546-4

[…] will naturally occur more often than other contacts of the same distance. there are a number of methods that apply this idea for hi-c, e.g. fit-hi-c (ay et al. ), homer (heinz et al. ) and hifive (sauria et al. ). for capture hi-c, so far, there is only chicago (cairns et al. ) that uses distance correction, taking into account the different properties of bait-bait and bait-non-bait […]

library_books

Targeted inhibition of oncogenic miR 21 maturation with designed RNA binding proteins

2016
Nat Chem Biol
PMCID: 4990487
PMID: 27428511
DOI: 10.1038/nchembio.2128

[…] the g. dicer and rrm*-dicer were inserted into a modified pfastbac1 vector (invitrogen) which has an n-terminal glutathione-s-transferase (gst) tag. the gst fusion proteins were expressed in hifive insect cells following standard procedures and isolated by glutathione affinity chromatography (ge healthcare: gstrap ff) using buffer containing 1×pbs and 5 mm dtt. after on-column cleavage […]

library_books

Serological Cross Reactivity between Merkel Cell Polyomavirus and Two Closely Related Chimpanzee Polyomaviruses

2014
PLoS One
PMCID: 4016208
PMID: 24816721
DOI: 10.1371/journal.pone.0097030

[…] of the polyhedrin promoter of pfastbac dual plasmid (invitrogen, fisherscientific, illkirch, france) and then used to generate recombinant baculoviruses using the bac-to-bac system (invitrogen). hifive cells maintained in grace medium (invitrogen) were infected with the different recombinant baculoviruses for production of the polyomavirus vlps. vlps were then purified by ultracentrifugation […]

library_books

Screening Compounds with a Novel High Throughput ABCB1 Mediated Efflux Assay Identifies Drugs with Known Therapeutic Targets at Risk for Multidrug Resistance Interference

2013
PLoS One
PMCID: 3622673
PMID: 23593196
DOI: 10.1371/journal.pone.0060334

[…] total number of cells counted per sample was 10,000., the interaction of selected compounds with abcb1 was assessed by an in vitro photolabeling assay as previously described . crude membranes from hifive cells overexpressing human abcb1 were incubated with the selected compounds for 5 minutes, after which 3 to 5 nmol/l [125i]iaap in 50 mm tris-hcl (ph 7.5) was added. after exposure to uv light […]

library_books

Seroprevalence and Cross reactivity of Human Polyomavirus 9

2012
PMCID: 3414022
PMID: 22840602
DOI: 10.3201/eid1808.111625

[…] piscataway, nj, usa) (genbank accession no. hq696595). we used the bac-to-bac baculovirus expression system (invitrogen, fisher scientific, illkirch, france) to generate recombinant baculoviruses. hifive cells maintained in grace medium (invitrogen) were infected with the different recombinant baculoviruses for the production of the different vlps. vlps were purified, and the presence of vlps […]


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HiFive institution(s)
Departments of Biology and Computer Science, Johns Hopkins University, Baltimore, MD, USA

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