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HiGene specifications

Information


Unique identifier OMICS_33805
Name HiGene
Software type Pipeline/Workflow
Interface Command line interface
Restrictions to use None
Operating system Unix/Linux
Computer skills Advanced
Stability Stable
Maintained Yes

Versioning


No version available

Maintainers


  • person_outline Liqun Deng
  • person_outline Jiansheng Wei

Publication for HiGene

HiGene citations

 (5)
library_books

CD163 as a novel target gene of STAT3 is a potential therapeutic target for gastric cancer

2017
Oncotarget
PMCID: 5675630
PMID: 29152078
DOI: 10.18632/oncotarget.20244

[…] was added 72 hours after infection at the concentration of 2 μg/mL (Sigma, P9620) until no dying cells were visible. For transfection assays, 2×105 cells were seeded into 6-wells and transfected with Higene (Applygen, C1506) following the manufacture's guidelines. Cells were washed with PBS and harvested with RIPA lysis buffer 48 hour after transfection. […]

library_books

A Novel Association between Lysyl Oxidase Gene Polymorphism and Intracranial Aneurysm in South Koreans

2017
PMCID: 5552627
PMID: 28792146
DOI: 10.3349/ymj.2017.58.5.1006

[…] II) from the LD TAG SNP Selection (TagSNP) of SNPinfo (https://snpinfo.niehs.nih.gov/). For genotyping of 10-tagged SNPs, genomic DNA from the peripheral blood of all 160 subjects was extracted using HiGene™ Genomic DNA Prep Kit (BIOFACT, Daejeon, Korea). Primers of ten SNPs were designed using the Primer-3 v.0.4.0 program (http://bioinfo.ut.ee/primer3-0.4.0/) (). Polymerase chain reaction (PCR) w […]

call_split

New Recorded Species in Three Genera of the Sordariomycetes in South Korea

2017
PMCID: 5541150
PMID: 28781538
DOI: 10.5941/MYCO.2017.45.2.64
call_split See protocol

[…] Total genomic DNA was extracted from isolates KNU16-001, KNU16-002, and KNU16-009 by using the HiGene Genomic DNA Prep Kit (BIOFACT, Daejeon, Korea), per manufacturer's instructions. The internal transcribed spacer (ITS) regions, including the 5.8S rRNA region, were amplified with primers ITS1 […]

library_books

miR 135a inhibits tumor metastasis and angiogenesis by targeting FAK pathway

2017
Oncotarget
PMCID: 5458197
PMID: 28415713
DOI: 10.18632/oncotarget.16098

[…] in (Sigma, USA) were added to select positive cells.In order to overexpress p53, 2 × 105 cells were seeded into 6-well plates, then 3μg pcDNA3.1flag-P53 or empty vector plasmids were transfected with Higene (Applygen, China) following the manufacturer's instruction. Cells were harvested 48 h after transfection, and total cell protein or RNA were extracted. For miRNA inhibitor transfection in trans […]

library_books

An analysis of indirect genetic effects on adult body weight of the Pacific white shrimp Litopenaeus vannamei at low rearing density

2015
PMCID: 4678730
PMID: 26667448
DOI: 10.1186/s12711-015-0164-y

[…] The breeding program was performed at Hainan Higene Aquaculture Technology Ltd (longitude 110.952874, latitude 19.937534) in Wenchang City, Hainan Province, China, in 2012. The founder broodstocks were from eight improved batches that were intro […]

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