High-resolution melt (HRM) curve analysis is a PCR-based assay that identifies sequence alterations based on subtle variations in the melting curves of mutated versus wild-type DNA sequences. HRM analysis is a high-throughput, sensitive, and efficient alternative to Sanger sequencing and is used to assess for mutations in clinically important genes involved in cancer diagnosis. The technique involves PCR amplification of a target sequence in the presence of a fluorescent double-stranded DNA (dsDNA) binding dye, melting of the fluorescent amplicons, and subsequent interpretation of melt curve profiles.
Calls the genotypes of single nucleotide polymorphisms (SNP) from TaqMan allelic discrimination assays. SNPman utilizes the fluorescence data collected over the whole polymerase chain reaction (PCR) run, rather than relying on the end point fluorescence measurements that is the basis of the genotype calling process in most software solutions sold with the real-time instruments. It works with data from three different widely used PCR instruments.
Detects variations in nucleic acid sequences. HRM is an application that can be run either locally or from the Thermo Fisher Cloud, with possible inter-operability among its two versions. It proposes a method that investigates polymerase chain reaction (PCR) melting curves to highlight the differences. The cloud application allows the simultaneous analysis of several plates whereas the desktop version offers an access to an additional set of data review tools.
Comprises a peak detection procedure and an ordinal regression model. McSNP is a relatively cheap and handy genotyping technique as it is an extension of the reverse transcription-polymerase chain reaction (RT-PCR) technique. It makes use of the difference in melting temperature of PCR products for different alleles. Users and clinicians can conduct the base calling using the default settings directly. A graphical user interface (GUI) is provided for the ease of data manipulation.
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