Who uses us

Workflow

High resolution melt software tools | PCR

High-resolution melt (HRM) curve analysis is a PCR-based assay that identifies sequence alterations based on subtle variations in the melting curves of mutated versus wild-type DNA sequences. HRM analysis is a high-throughput, sensitive, and efficient alternative to Sanger sequencing and is used to assess for mutations in clinically important genes involved in cancer diagnosis. The technique involves PCR amplification of a target sequence in the presence of a fluorescent double-stranded DNA (dsDNA) binding dye, melting of the fluorescent amplicons, and subsequent interpretation of melt curve profiles.
1 - 7 of 7 results
tune Filters / Sort by
Sort by:
Filter by:
Desktop filters Mobile filters

Want to access data filter options?

Get started
1 - 7 of 7 results