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HYDEN | The degenerate primer design problem


A program for designing pairs of degenerate primers for a given set of DNA sequences. We report on the success of the program in an experimental scheme for identifying all human olfactory receptor (OR) genes. In that project, HYDEN was used to design primers with degeneracies up to 10(10) that amplified with high specificity many novel genes of that family, tripling the number of OR genes known at the time.

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HYDEN classification

HYDEN specifications

Unique identifier:
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HighlY DEgeNerate primers
Command line interface
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HYDEN distribution


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School Of Computer Science, Tel-Aviv University, Ramat-Aviv, Tel-Aviv, Israel

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