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HIrisPlex specifications


Unique identifier OMICS_26232
Name HIrisPlex
Interface Web user interface
Restrictions to use None
Input data A file containing the number of minor alleles of the 24 assay DNA variants.
Input format CSV
Computer skills Basic
Stability Stable
Maintained Yes



  • person_outline Manfred Kayser

Publications for HIrisPlex

HIrisPlex citations


Population genomic analysis of elongated skulls reveals extensive female biased immigration in Early Medieval Bavaria

Proc Natl Acad Sci U S A
PMCID: 5879695
PMID: 29531040
DOI: 10.1073/pnas.1719880115

[…] (SI Appendix, section 7), many of which are thought to have changed frequencies in human populations over the last 10,000 y due to adaptation to different environments and changing diet. Based on the HIrisPlex system (), the majority (∼80%) of individuals with normal or intermediate skulls (and thus northern/central European ancestry) showed high probabilities for blue eyes and blonde hair (SI App […]


Predicting hair cortisol levels with hair pigmentation genes: a possible hair pigmentation bias

Sci Rep
PMCID: 5561185
PMID: 28819144
DOI: 10.1038/s41598-017-07034-w

[…] Hardy-Weinberg equilibrium (p < 10−7). Excess heterozygosity, gender accuracy, and relatedness were tested. We used MACH 1.0 to impute to the 1000 Genomes Iv3 reference .We selected 22 SNPs from the HIrisPlex System related to hair color prediction. Nine SNPs were directly genotyped in Generation R and 13 were available as imputed genotypes. Of these, 3 were excluded due to poor imputation qualit […]


Genetic origins of the Minoans and Mycenaeans

PMCID: 5565772
PMID: 28783727
DOI: 10.1038/nature23310
call_split See protocol

[…] ior distribution of the genotypes g given the read counts r of the reference allele and t of the total reads covering a site. We took 100 random genotype samples per individuals and submitted them to HIrisPlex, obtaining an estimate of the uncertainty of phenotype inference (, section 4; ). […]


Paleogenomic Evidence for Multi generational Mixing between Neolithic Farmers and Mesolithic Hunter Gatherers in the Lower Danube Basin

Curr Biol
PMCID: 5483232
PMID: 28552360
DOI: 10.1016/j.cub.2017.05.023

[…] 0 and positions covered by at least 3 reads. Owing to the low coverage of some of the genomes, the identification of the genotype as homozygous or heterozygous was inconclusive at some positions. The Hirisplex [] and 8-plex [] prediction systems were used to predict eye, hair and skin color in the ancient samples. Furthermore, we also investigated loci related to lactase persistence.We used imputa […]


Global skin colour prediction from DNA

Hum Genet
PMCID: 5487854
PMID: 28500464
DOI: 10.1007/s00439-017-1808-5

[…] al. , ; Valenzuela et al. ; Valverde et al. ; Voisey et al. ), 22 of which proved decidedly predictive for hair colour categories (Branicki et al. ). From this, and previous eye colour knowledge, the HIrisPlex system was developed and forensically validated for combined eye and hair colour prediction from DNA achieving AUCs of 0.92 for red, 0.85 for black, 0.81 for blond, and 0.75 for brown (Draus […]


Novel quantitative pigmentation phenotyping enhances genetic association, epistasis, and prediction of human eye colour

Sci Rep
PMCID: 5327401
PMID: 28240252
DOI: 10.1038/srep43359

[…] ingle multiplex assay targeting 12-eye colour SNPs. We used 6 SNPs that were previously identified to predict categorical eye colour in genome wide studies) and which are included in the IrisPlex and HIrisPlex DNA test systems for categorical eye colour prediction: HERC2 rs12913832, OCA2 rs1800407, SLC24A4 rs12896399, SLC45A2 (MATP) rs16891982, TYR rs1393350, and IRF4 rs12203592. The 6 additional […]


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HIrisPlex institution(s)
Department of Forensic Molecular Biology, Erasmus MC University Medical Centre Rotterdam, Rotterdam, The Netherlands; Department of Human Biological Traces, Netherlands Forensic Institute, The Hague, The Netherlands; Forensic Laboratory for DNA Research, Department of Human Genetics, Leiden University Medical Center, Leiden, The Netherlands; Section of Forensic Genetics, Institute of Forensic Research, Krakow, Poland; Laboratory of Forensic Medicine & Toxicology, School of Medicine, Aristotle University of Thessaloniki, Thessaloniki, Greece; Department of Legal Medicine, Osaka City University, Medical School, Osaka, Japan; Division of Legal Medicine, Faculty of Medicine, Tottori University, Japan; Department of Genetics and Evolution, Institute of Zoology, Jagiellonian University, Krakow, Poland
HIrisPlex funding source(s)
Supported in part by the Netherlands Forensic Institute (NFI), Erasmus MC University Medical Center Rotterdam, a grant from the Netherlands Genomics Initiative (NGI)/ Netherlands Organization for Scientific Research (NWO) within the framework of the Forensic Genomics Consortium Netherlands (FGCN) and support from the European Union Seventh Framework Programme (FP7/2007–2013) under grant agreement no 285487 (EUROFORGEN-NoE).

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