Tool stats & trends
Looking to identify usage trends or leading experts?
|Alternative name||High-Througput Insertion Site Analysis Pipeline|
|Interface||Web user interface|
|Restrictions to use||None|
Publication for High-Througput Insertion Site Analysis Pipeline
Genome wide Specificity of Highly Efficient TALENs and CRISPR/Cas9 for T Cell Receptor Modification
[…] platform, barcode-containing adapters were added to LAM-PCR products by an additional PCR step, and the integration site data were analyzed using the high-throughput insertion site analysis pipeline (HISAP). Additional in silico off-target prediction for βC1 and βC3 was accomplished with the web-based COSMID tool. For all analyses, the UCSC Assembly hg19 (February 2009) was used as reference genom […]
GENE IS: Time Efficient and Accurate Analysis of Viral Integration Events in Large Scale Gene Therapy Data
[…] (). The advent of high-throughput sequencing technologies has revolutionized the possibilities of in-depth genome analyses. However, computational tools available for LAM IS analysis, like QuickMap, HISAP, and VISA, have issues with time efficiency and are not offering targeted sequencing IS analysis. Besides, these tools admit a limited set of reference genomes, do not expose multi-threading, an […]
Comparison of Lentiviral and Sleeping Beauty Mediated αβ T Cell Receptor Gene Transfer
[…] the second round primers. LM-PCR products were 454 pyrosequenced as previously described .Raw sequence reads obtained after sequencing were trimmed and aligned to the human genome (NCBI, hg19) using HISAP . Integration sites were considered to be valid if vector-genome junction sequence was present and the flanking genomic region had a unique sequence match of at least 95% after alignment to the […]
Looking to check out a full list of citations?
Be the first to review HISAP