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HKL-2000 specifications


Unique identifier OMICS_29328
Name HKL-2000
Alternative name HKL2000
Software type Package/Module
Interface Graphical user interface
Restrictions to use None
Operating system Unix/Linux, Mac OS
Computer skills Medium
Maintained Yes


No version available


  • person_outline Wladek Minor
  • person_outline HKL-2000 Team
  • person_outline Zbyszek Otwinowski

Publication for HKL-2000

HKL-2000 citations


Structure of a cleavage independent HIV Env recapitulates the glycoprotein architecture of the native cleaved trimer

Nat Commun
PMCID: 5955915
PMID: 29769533
DOI: 10.1038/s41467-018-04272-y

[…] X-ray diffraction data processing (integration and scaling) was performed with HKL-2000. The crystal structure of unbound PGV19 was solved by MR using PGV20 (PDB 4LSU) as the search model using Phaser. The BG505 NFL.664+PGV19+PGT122 complex structure was determined by MR with PG […]


Functional insights into the interplay between DNA interaction and metal coordination in ferric uptake regulators

Sci Rep
PMCID: 5940780
PMID: 29739988
DOI: 10.1038/s41598-018-25157-6
call_split See protocol

[…] harvested and soaked in the mother liquor supplemented with 7.5% glycerol. A full dataset was collected at the 17-ID beamline at Argonne National Laboratory’s Advanced Photon Source and indexed using HKL-2000 (HKL Research). The structure of CjFurZn was solved by molecular replacement using the CjFur DBD and DD (PDB: 4ETS) as search models and the program Phaser from the PHENIX package. The model […]


Identification of the I38T PA Substitution as a Resistance Marker for Next Generation Influenza Virus Endonuclease Inhibitors

PMCID: 5915737
PMID: 29691337
DOI: 10.1128/mBio.00430-18

[…] ution supplemented with 30% glycerol. X-ray diffraction data were collected at the SERCAT 22-ID or 22-BM beam lines at the Advanced Photon Source. Data sets were indexed, integrated, and scaled using HKL-2000 (), and the structures were determined by molecular replacement using Phaser (). Model building and refinement were done using Phenix, CCP4, and coot. For data collection and refinement stati […]


Structures of the Mycobacterium tuberculosis GlpX protein (class II fructose 1,6 bisphosphatase): implications for the active oligomeric state, catalytic mechanism and citrate inhibition

Acta Crystallogr D Struct Biol
PMCID: 5892879
PMID: 29652259
DOI: 10.1107/S2059798318002838
call_split See protocol

[…] the crystal-to-detector distance was set to 280 mm and 200 frames were collected with a 1° scan width and 7.3 s exposure time.The diffraction data were integrated, reduced, processed and scaled with HKL-2000 (Otwinowski & Minor, 1997). The structure solutions for all crystal forms were obtained by molecular replacement using a partially refined structure of the apo enzyme as a model. This initial […]


The putative compatible solute binding protein ProX from Mycobacterium tuberculosis H37Rv: biochemical characterization and crystallographic data

PMCID: 5894108
PMID: 29633971
DOI: 10.1107/S2053230X18003771

[…] llected at 100 K in a liquid-nitrogen stream using an IµS 3.0 microfocus source with a PHOTON II detector (Bruker) at Lanzhou University. All diffraction data were indexed, integrated and scaled with HKL-2000 (Otwinowski & Minor, 1997). Data-collection statistics are summarized in Table 3. […]


Structure of Schlafen13 reveals a new class of tRNA/rRNA targeting RNase engaged in translational control

Nat Commun
PMCID: 5862951
PMID: 29563550
DOI: 10.1038/s41467-018-03544-x
call_split See protocol

[…] n Facility (SSRF), and one data set of SeMet-substituted rSLFN1314–353(M176A) at the selenium peak wavelength was collected at beamline BL17U1 of SSRF. Data sets were processed with the XDS suite and HKL-2000 programme suite. Initial phases were obtained by the single-anomalous dispersion method and refined using Phaser. In the calculated electron density, the main chains and many side chains were […]


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