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Performs annotation of carbohydrate-active enzymes and allows prediction of the enzymatic activity of the proteins. Hotpep (Homology to Peptide Pattern) is a state-of-the-art method for automatic annotation and functional prediction. The software matches the short, conserved motifs to undescribed protein sequences to obtain a fast, sensitive and precise annotation of carbohydrate-active enzymes to families. It also provides a functional prediction of function directly from amino acid sequence.

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Hotpep classification

Hotpep specifications

Software type:
Restrictions to use:
Input format:
Operating system:
Computer skills:
Command line interface
Input data:
A text file with predicted protein sequences (for annotation).
Output data:
One directory for each enzyme class in the CAZy database, a file containing a summary of the results and a file with all the families found for each accession number. Each of the class directories contains files with the hits for each family, a summary and a directory with functional predictions. The folder with functional predictions contains files for each EC number found and a summary.
Programming languages:

Hotpep distribution


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Hotpep support



  • Pete Busk <>


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Department of Chemical and Biochemical Engineering, Technical University of Denmark, Soltofts Plads, Lyngby, Denmark

Funding source(s)

Supported by project number Mar 14319 from Nordic Innovation, SYNFERON – from the Danish Innovation Fund and by The Villum Foundation.

Link to literature

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