HotSpot Wizard statistics

To access cutting-edge analytics on consensus tools, life science contexts and associated fields, you will need to subscribe to our premium service.


Citations per year

Citations chart

Popular tool citations

chevron_left Protein design Hot spot prediction Annotation chevron_right
Popular tools chart

Tool usage distribution map

Tool usage distribution map

Associated diseases

Associated diseases

HotSpot Wizard specifications


Unique identifier OMICS_08151
Name HotSpot Wizard
Interface Web user interface
Restrictions to use None
Input data Tertiary structure of the query protein
Input format PDB
Output data hot spots
Output format ZIP
Computer skills Basic
Version 2.0
Stability Stable
Maintained Yes



  • person_outline HotSpot Wizard <>

Publications for HotSpot Wizard

HotSpot Wizard in publications

PMCID: 5734778
PMID: 29253895
DOI: 10.1371/journal.pone.0189806

[…] pymol 0.99rc was employed to visualize the homology model and structure alignment. the catalytic amino acid residues and amino acid frequency in the swiss-prot database were predicted by hotspot wizard [] ( amino acid interactions were predicted by residue interaction network generator (ring) web server [] […]

PMCID: 5681632
PMID: 29127292
DOI: 10.1038/s41598-017-12659-y

[…] structure 3wp3 as template, modeled tlxyn11b showed a β-jelly roll structure of typical gh11 xylanases and an extended n-terminus at the bottom of the palm region (supplementary fig. ). based on the hotspot wizard analysis, two conserved glutamates (94 and 185) were suggested to be catalytic residues. energy minimization analysis of modeled tlxyn11b by chimera 1.10 […]

PMCID: 5500549
PMID: 28684739
DOI: 10.1038/s41598-017-02578-3

[…] were made computationally, and alterations to the protein structure were calculated using modeller 9.11.figure 3 table 1 (a)the mutant residue types and frequencies were statistically analyzed using hotspot wizard 1.7 software based on the results of the multiple sequence alignment. , (a)the mutant residue types and frequencies were statistically analyzed using hotspot wizard 1.7 software based […]

PMCID: 3962226
PMID: 24688657
DOI: 10.5936/csbj.201209016

[…] which may not be present in experimentally oriented research groups. therefore, online tools that require little to none bioinformatics knowledge have become popular. meta-tools such as the hotspot wizard [] offer a complete workflow to assess promising mutation sites by combining a variety of methods such as catalytic site atlas [], castp [], caver [], blast [], muscle [], as well […]

To access a full list of publications, you will need to upgrade to our premium service.

HotSpot Wizard institution(s)
Loschmidt Laboratories, Department of Experimental Biology and Research Centre for Toxic Compounds in the Environment RECETOX, Masaryk University, Brno, Czech Republic; Department of Information Systems, Faculty of Information Technology, Brno University of Technology, Brno, Czech Republic; International Clinical Research Center, St. Anne’s University Hospital Brno, Brno, Czech Republic
HotSpot Wizard funding source(s)
Supported by Ministry of Education of the Czech Republic [LO1214, LQ1605, LM2015055 and LM2015047]; Grant Agency of the Czech Republic [GA16-06096S]; European Commission REGPOT [316345]; Horizon 2020 Research Infrastructure ELIXIR-EXCELERATE [676559]; Brno University of Technology [FIT-S-14-2299 to M.M.].

HotSpot Wizard reviews

star_border star_border star_border star_border star_border
star star star star star

Be the first to review HotSpot Wizard