HTP-OligoDesigner statistics

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HTP-OligoDesigner specifications

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Information

Unique identifier OMICS_11468
Name HTP-OligoDesigner
Interface Web user interface
Restrictions to use None
Input data HTP-OligoDesigner is capable of designing primers for three types of mutations: point mutation, deletion, and insertion. Desired mutations must be indicated into each sequence so that they can be joined in a flat text or uploaded text file in FASTA format.
Output data Output data from both mutagenesis and cloning tools are given as plain text containing the GeneID (first uninterrupted characters from FASTA header), primers sequences, lengths, and Tm values.
Programming languages Javascript
Computer skills Basic
Stability Stable
Maintained Yes

Maintainer

  • person_outline Cesar M. Camilo <>

Publication for HTP-OligoDesigner

HTP-OligoDesigner in publications

 (2)
library_books

Structure, computational and biochemical analysis of PcCel45A endoglucanase from Phanerochaete chrysosporium and catalytic mechanisms of GH45 subfamily C members

PMCID: 5829257
PMID: 29487297
DOI: 10.1038/s41598-018-21798-9

[…] on interactions between pccel45a and cellobiose. residues tyr18, tyr67, asp85, asp114 and trp154 were mutated to alanines using a site-directed mutagenesis technique and primers were designed with htp-oligodesigner. procedures were based on an inverse polymerase chain reaction (pcr) method in which the entire plasmid dna is amplified by pcr. details of the protocol, primers and vectors […]

library_books

Molecular characterization of a family 5 glycoside hydrolase suggests an induced fit enzymatic mechanism

PMCID: 4817029
PMID: 27032335
DOI: 10.1038/srep23473

[…] dna polymerase (neb, usa) was used for amplifications with the plasmid pettrxa-1a/lic-blcel5b as a template. mutagenic primers blcel5bw479a_fw/rv and blcel5bw481a_fw/rv () were generated by htp-oligodesigner tool (http://www.ifsc.usp.br/htpoligo/)., enzymatic activity assays were performed by a colorimetric method using the 3,5-dinitrosalicylic acid (dns), with glucose being a standard […]


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HTP-OligoDesigner institution(s)
Grupo de Biotecnologia Molecular, Instituto de Física de São Carlos, Universidade de São Paulo, São Carlos, São Paulo, Brazil; Laboratório de Química Medicinal e Computacional, Centro de Inovação em Biodiversidade e Fármacos, Instituto de Física de São Carlos, Universidade de São Paulo, São Carlos, São Paulo, Brazil

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