HTP-OligoDesigner statistics

info info

Citations per year

Number of citations per year for the bioinformatics software tool HTP-OligoDesigner
info

Tool usage distribution map

This map represents all the scientific publications referring to HTP-OligoDesigner per scientific context
info info

Associated diseases

info

Popular tool citations

chevron_left Mutagenic primer design chevron_right
Want to access the full stats & trends on this tool?

HTP-OligoDesigner specifications

Information


Unique identifier OMICS_11468
Name HTP-OligoDesigner
Interface Web user interface
Restrictions to use None
Input data HTP-OligoDesigner is capable of designing primers for three types of mutations: point mutation, deletion, and insertion. Desired mutations must be indicated into each sequence so that they can be joined in a flat text or uploaded text file in FASTA format.
Output data Output data from both mutagenesis and cloning tools are given as plain text containing the GeneID (first uninterrupted characters from FASTA header), primers sequences, lengths, and Tm values.
Programming languages Javascript
Computer skills Basic
Stability Stable
Maintained Yes

Maintainer


  • person_outline Cesar M. Camilo

Publication for HTP-OligoDesigner

HTP-OligoDesigner citations

 (3)
library_books

Structure, computational and biochemical analysis of PcCel45A endoglucanase from Phanerochaete chrysosporium and catalytic mechanisms of GH45 subfamily C members

2018
Sci Rep
PMCID: 5829257
PMID: 29487297
DOI: 10.1038/s41598-018-21798-9

[…] ed on interactions between PcCel45A and cellobiose. Residues Tyr18, Tyr67, Asp85, Asp114 and Trp154 were mutated to alanines using a site-directed mutagenesis technique and primers were designed with HTP-OligoDesigner. Procedures were based on an inverse polymerase chain reaction (PCR) method in which the entire plasmid DNA is amplified by PCR. Details of the protocol, primers and vectors are give […]

library_books

High throughput recombinant protein expression in Escherichia coli: current status and future perspectives

2016
Open Biol
PMCID: 5008019
PMID: 27581654
DOI: 10.1098/rsob.160196

[…] any effort to express recombinant proteins (b). After genes of interest have been selected, a batch of primers can be designed based on the coding sequences using online tools such as PrimerCE [] and HTP-OligoDesigner []. High-throughput PCR and PCR-product purification are now mature technologies that can be completed using automated laboratory workstations []. However, problems such as the absen […]

library_books

Molecular characterization of a family 5 glycoside hydrolase suggests an induced fit enzymatic mechanism

2016
Sci Rep
PMCID: 4817029
PMID: 27032335
DOI: 10.1038/srep23473

[…] Fidelity” DNA polymerase (NEB, USA) was used for amplifications with the plasmid pETTRXA-1a/LIC-Blcel5B as a template. Mutagenic primers Blcel5BW479A_Fw/Rv and Blcel5BW481A_Fw/Rv () were generated by HTP-OligoDesigner tool (http://www.ifsc.usp.br/htpoligo/). […]


Want to access the full list of citations?
HTP-OligoDesigner institution(s)
Grupo de Biotecnologia Molecular, Instituto de Física de São Carlos, Universidade de São Paulo, São Carlos, São Paulo, Brazil; Laboratório de Química Medicinal e Computacional, Centro de Inovação em Biodiversidade e Fármacos, Instituto de Física de São Carlos, Universidade de São Paulo, São Carlos, São Paulo, Brazil

HTP-OligoDesigner reviews

star_border star_border star_border star_border star_border
star star star star star

Be the first to review HTP-OligoDesigner