HTP-OligoDesigner statistics
HTP-OligoDesigner specifications
Information
Unique identifier | OMICS_11468 |
---|---|
Name | HTP-OligoDesigner |
Interface | Web user interface |
Restrictions to use | None |
Input data | HTP-OligoDesigner is capable of designing primers for three types of mutations: point mutation, deletion, and insertion. Desired mutations must be indicated into each sequence so that they can be joined in a flat text or uploaded text file in FASTA format. |
Output data | Output data from both mutagenesis and cloning tools are given as plain text containing the GeneID (first uninterrupted characters from FASTA header), primers sequences, lengths, and Tm values. |
Programming languages | Javascript |
Computer skills | Basic |
Stability | Stable |
Maintained | Yes |
Maintainer
- person_outline Cesar M. Camilo <>
Publication for HTP-OligoDesigner
HTP-OligoDesigner: An Online Primer Design Tool for High-Throughput Gene Cloning and Site-Directed Mutagenesis.
HTP-OligoDesigner in publications
(2)[…] on interactions between pccel45a and cellobiose. residues tyr18, tyr67, asp85, asp114 and trp154 were mutated to alanines using a site-directed mutagenesis technique and primers were designed with htp-oligodesigner. procedures were based on an inverse polymerase chain reaction (pcr) method in which the entire plasmid dna is amplified by pcr. details of the protocol, primers and vectors […]
Molecular characterization of a family 5 glycoside hydrolase suggests an induced fit enzymatic mechanism
[…] dna polymerase (neb, usa) was used for amplifications with the plasmid pettrxa-1a/lic-blcel5b as a template. mutagenic primers blcel5bw479a_fw/rv and blcel5bw481a_fw/rv () were generated by htp-oligodesigner tool (http://www.ifsc.usp.br/htpoligo/)., enzymatic activity assays were performed by a colorimetric method using the 3,5-dinitrosalicylic acid (dns), with glucose being a standard […]
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