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HTP-OligoDesigner specifications
Information
| Unique identifier | OMICS_11468 |
|---|---|
| Name | HTP-OligoDesigner |
| Interface | Web user interface |
| Restrictions to use | None |
| Input data | HTP-OligoDesigner is capable of designing primers for three types of mutations: point mutation, deletion, and insertion. Desired mutations must be indicated into each sequence so that they can be joined in a flat text or uploaded text file in FASTA format. |
| Output data | Output data from both mutagenesis and cloning tools are given as plain text containing the GeneID (first uninterrupted characters from FASTA header), primers sequences, lengths, and Tm values. |
| Programming languages | Javascript |
| Computer skills | Basic |
| Stability | Stable |
| Maintained | Yes |
Maintainer
- person_outline Cesar M. Camilo
Publication for HTP-OligoDesigner
HTP-OligoDesigner: An Online Primer Design Tool for High-Throughput Gene Cloning and Site-Directed Mutagenesis.
HTP-OligoDesigner citations
(3)Structure, computational and biochemical analysis of PcCel45A endoglucanase from Phanerochaete chrysosporium and catalytic mechanisms of GH45 subfamily C members
[…] ed on interactions between PcCel45A and cellobiose. Residues Tyr18, Tyr67, Asp85, Asp114 and Trp154 were mutated to alanines using a site-directed mutagenesis technique and primers were designed with HTP-OligoDesigner. Procedures were based on an inverse polymerase chain reaction (PCR) method in which the entire plasmid DNA is amplified by PCR. Details of the protocol, primers and vectors are give […]
High throughput recombinant protein expression in Escherichia coli: current status and future perspectives
[…] any effort to express recombinant proteins (b). After genes of interest have been selected, a batch of primers can be designed based on the coding sequences using online tools such as PrimerCE [] and HTP-OligoDesigner []. High-throughput PCR and PCR-product purification are now mature technologies that can be completed using automated laboratory workstations []. However, problems such as the absen […]
Molecular characterization of a family 5 glycoside hydrolase suggests an induced fit enzymatic mechanism
[…] Fidelity” DNA polymerase (NEB, USA) was used for amplifications with the plasmid pETTRXA-1a/LIC-Blcel5B as a template. Mutagenic primers Blcel5BW479A_Fw/Rv and Blcel5BW481A_Fw/Rv () were generated by HTP-OligoDesigner tool (http://www.ifsc.usp.br/htpoligo/). […]
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