Huygens

[…] eclipse ti-e inverted microscope equipped with a 60 × planapo vc oil objective na 1.40 (or 40 × planfluor objective with na 0.75 and 1.5 zoom). image stacks were automatically deconvolved using the huygens batch processing application (scientific volume imaging) and deconvolved image stacks were transformed into maximum projection images using custom matlab scripts. cell segmentation and ld […]

[…] during the long movies (). as a result, particles that entered the nucleus from the top or bottom were not detected. deconvolution was applied to the images to remove the out of focus noise using huygens software (svi, netherlands). the lateral movement of the nucleus during each movie was corrected using the pom121-mcherry signal and a custom-written matlab program (). after adjusting […]

[…] from two adjoining optical slices. next, we used macro hyprbkgdfix to clear background outside the stained tissue to facilitate 3d volume visualization. finally, the 3d images were deconvolved using huygens pro software v. 4.3 (scientific volume imaging), to correct for refractive index mismatch, and to enhance spatial signals and cell morphology discrimination. we constructed 3d tomographic […]

[…] contrast of individual channels were adjusted in imagej (nih) after cropping the area of interest. the sted preexpansion images shown in (first and third panels) and used in were deconvolved using huygens (scientific volume imaging). tracing of cellular processes (shown in ) was performed using imaris. this tracing algorithm is intensity-based. first, start and end points are detected, […]

[…] and analyzed using metamorph software (molecular devices). optical section data (41 focal planes with 0.2 μm spacing every 30 sec.) were collected. time-lapse sequences were deconvolved using huygens image analysis software (scientific volume imaging)., microscope images of the interphase cells were used to examine the distance between spb and laco-labeled genomic loci. deconvolved time […]

[…] microscope (leica microsystems) maintained by the center for microscopy and image analysis, university of zurich. to visualise the localisation of mxb in t98g cells, deconvolution was applied using huygens version 17.04., t98g cells were treated with 500 iu/ml recombinant human ifn-α2 (roferon-a, roche pharmaceuticals) 18 h prior to virus infection. during and after virus inoculation, the ifn […]

[…] control and experimental samples were imaged with the same laser setting. for the f-actin images in mouse tissue (fig.  and supplementary fig. ), confocal images were deconvoluted using huygens software., rna was isolated from 20-day-old drosophila brains (16 brains per sample) using qiazol (qiagen) and reverse-transcribed with the high-capacity cdna reverse transcription kit […]

[…] z-series images were acquired at 0.15 μm increments through the entire visible dendrite. automated image analysis was performed as described []. briefly, z-stack image series were deconvolved in huygens using the blind algorithm that employs an iteratively refined theoretical point spread function. for preparation of figures, maximum intensity z-projections were generated in imaris. […]

[…] (nikon), ds-qi2 cmos camera (nikon), and 37 °c, 5% co2 heat stage (live cell instrument). imaging was acquired using nikon element ar software. images with multiple z-stacks were processed with huygens deconvolution (scientific volume imaging), and the maximum intensity projections of images were produced by nikon element ar software (nikon). the image analysis was mainly conducted by nikon […]

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