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Huygens specifications

Information


Unique identifier OMICS_18438
Name Huygens
Software type Package/Module
Interface Graphical user interface
Restrictions to use License purchase required
Operating system Unix/Linux
Computer skills Medium
Version 17.04
Stability Stable
Maintained Yes

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  • person_outline Huygens Team <>

Huygens in pipelines

 (24)
2017
PMCID: 5380971
PMID: 28361956
DOI: 10.1038/ncomms14858

[…] eclipse ti-e inverted microscope equipped with a 60 × planapo vc oil objective na 1.40 (or 40 × planfluor objective with na 0.75 and 1.5 zoom). image stacks were automatically deconvolved using the huygens batch processing application (scientific volume imaging) and deconvolved image stacks were transformed into maximum projection images using custom matlab scripts. cell segmentation and ld […]

2017
PMCID: 5578721
PMID: 28827840
DOI: 10.1371/journal.ppat.1006570

[…] during the long movies (). as a result, particles that entered the nucleus from the top or bottom were not detected. deconvolution was applied to the images to remove the out of focus noise using huygens software (svi, netherlands). the lateral movement of the nucleus during each movie was corrected using the pom121-mcherry signal and a custom-written matlab program (). after adjusting […]

2017
PMCID: 5717053
PMID: 29208908
DOI: 10.1038/s41598-017-16987-x

[…] from two adjoining optical slices. next, we used macro hyprbkgdfix to clear background outside the stained tissue to facilitate 3d volume visualization. finally, the 3d images were deconvolved using huygens pro software v. 4.3 (scientific volume imaging), to correct for refractive index mismatch, and to enhance spatial signals and cell morphology discrimination. we constructed 3d tomographic […]

2017
PMCID: 5740639
PMID: 29162696
DOI: 10.1073/pnas.1706281114

[…] contrast of individual channels were adjusted in imagej (nih) after cropping the area of interest. the sted preexpansion images shown in (first and third panels) and used in were deconvolved using huygens (scientific volume imaging). tracing of cellular processes (shown in ) was performed using imaris. this tracing algorithm is intensity-based. first, start and end points are detected, […]

2016
PMCID: 4700429
PMID: 26729303
DOI: 10.1038/srep18757

[…] and analyzed using metamorph software (molecular devices). optical section data (41 focal planes with 0.2 μm spacing every 30 sec.) were collected. time-lapse sequences were deconvolved using huygens image analysis software (scientific volume imaging)., microscope images of the interphase cells were used to examine the distance between spb and laco-labeled genomic loci. deconvolved time […]


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Huygens in publications

 (980)
PMCID: 5958057
PMID: 29773792
DOI: 10.1038/s41467-018-04379-2

[…] microscope (leica microsystems) maintained by the center for microscopy and image analysis, university of zurich. to visualise the localisation of mxb in t98g cells, deconvolution was applied using huygens version 17.04., t98g cells were treated with 500 iu/ml recombinant human ifn-α2 (roferon-a, roche pharmaceuticals) 18 h prior to virus infection. during and after virus inoculation, the ifn […]

PMCID: 5954157
PMID: 29765022
DOI: 10.1038/s41467-018-04269-7

[…] control and experimental samples were imaged with the same laser setting. for the f-actin images in mouse tissue (fig.  and supplementary fig. ), confocal images were deconvoluted using huygens software., rna was isolated from 20-day-old drosophila brains (16 brains per sample) using qiazol (qiagen) and reverse-transcribed with the high-capacity cdna reverse transcription kit […]

PMCID: 5928584
PMID: 29716652
DOI: 10.1186/s40478-018-0537-x

[…] z-series images were acquired at 0.15 μm increments through the entire visible dendrite. automated image analysis was performed as described []. briefly, z-stack image series were deconvolved in huygens using the blind algorithm that employs an iteratively refined theoretical point spread function. for preparation of figures, maximum intensity z-projections were generated in imaris. […]

PMCID: 5928066
PMID: 29712905
DOI: 10.1038/s41467-018-03952-z

[…] (nikon), ds-qi2 cmos camera (nikon), and 37 °c, 5% co2 heat stage (live cell instrument). imaging was acquired using nikon element ar software. images with multiple z-stacks were processed with huygens deconvolution (scientific volume imaging), and the maximum intensity projections of images were produced by nikon element ar software (nikon). the image analysis was mainly conducted by nikon […]

PMCID: 5910469
PMID: 29706747
DOI: 10.1186/s13660-018-1687-x

[…] {\oddsidemargin}{-69pt} \begin{document}$x\in ( 0,+\infty ) $\end{document}x∈(0,+∞)., so the proof of theorem  is complete. □, the inequalities () and () are obviously better than the famous cusa–huygens inequality (see [–]): 5.1\documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usep […]


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