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HX-Express specifications


Unique identifier OMICS_06892
Name HX-Express
Alternative name HX-Express2
Software type Package/Module
Interface Command line interface
Restrictions to use None
Operating system Unix/Linux
License GNU General Public License version 2.0
Computer skills Advanced
Version 2.0
Stability Stable
Maintained Yes


No version available

Publications for HX-Express

HX-Express citations


Substrate modulated unwinding of transmembrane helices in the NSS transporter LeuT

Sci Adv
PMCID: 5947982
PMID: 29756037
DOI: 10.1126/sciadv.aar6179

[…] lay a measurable difference in HDX between the 60-min time point and the equilibrium-labeled control sample.Peptides exhibiting bimodal isotopic envelopes upon deuteration were further analyzed using HX-Express 2.0. A brief description of the analysis procedure for peptides displaying EX1 kinetics can be found in the Supplementary Materials. […]


Structural Dynamics of the GW182 Silencing Domain Including its RNA Recognition motif (RRM) Revealed by Hydrogen Deuterium Exchange Mass Spectrometry

J Am Soc Mass Spectrom
PMCID: 5785596
PMID: 29080206
DOI: 10.1007/s13361-017-1830-9
call_split See protocol

[…] data were verified by visual inspection and exported to Excel, R, or Prism 6 (GraphPad) for further analyses. Bimodal isotopic envelopes were exported directly from MassLynx (Waters) and analyzed by HX-Express2 [].The fraction of peptide amide hydrogens exchanged to deuterium, D f, for each peptide was calculated as:1Df=Mt−M¯inM¯bx−M¯inwhere M t – the average mass of the peptide after a given exc […]


Bacterial flagellar capping proteins adopt diverse oligomeric states

PMCID: 5072837
PMID: 27664419
DOI: 10.7554/eLife.18857.025
call_split See protocol

[…] sted to percent deuteration using the fully deuterated controls. Confidence intervals (98%) were plotted on the Δ%D plots as horizontal dashed lines. EX1 type cooperative unfolding was analyzed using HX-Express2 (). […]


DnaC traps DnaB as an open ring and remodels the domain that binds primase

Nucleic Acids Res
PMCID: 4705694
PMID: 26420830
DOI: 10.1093/nar/gkv961

[…] on in positive-ion mode. Mass spectra were acquired in continuum mode over m/z 50–2000 at a mass resolution (M/ΔM) of approximately 20 000 (full width-half maximum). Data analysis was performed using HX Express software and MS tools (,).As a control and also to determine the identities of undeuterated peptides, DnaB or DnaC (3 μg each, respectively) in 10 μl of Buffer A supplemented with 2 mM ATP […]


Considerably Unfolded Transthyretin Monomers Preceed and Exchange with Dynamically Structured Amyloid Protofibrils

Sci Rep
PMCID: 4480009
PMID: 26108284
DOI: 10.1038/srep11443

[…] 5 kV, cone voltage 40 V, and ion source block temperature 120 °C with a desolvation gas flow of 500 l/h at 200 °C and nebulising gas flow of 20 l/h at room temperature. The HXMS data were analysed by HX-Express. To identify peptic peptides, non-deuterated protein was digested and desalted as described above. The peptic peptides were eluted and collected for nanoflow-LC-MS/MS analysis, which was ca […]


Cyclic nucleotide binding and structural changes in the isolated GAF domain of Anabaena adenylyl cyclase, CyaB2

PMCID: 4411481
PMID: 25922789
DOI: 10.7717/peerj.882
call_split See protocol

[…] tion experiments using prototype custom software (HDX browser, Waters, Milford, Massachusetts, USA). Data on each individual peptide at all periods were extracted using this software, and exported to HX-Express () for analysis. A total number of 38 peptide fragments yielded primary sequence coverage of 96%. Continuous instrument calibration was carried out with Glu-fibrinogen peptide at 100 fmol/µ […]


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HX-Express institution(s)
Department of Medicinal Chemistry, University of Washington, Seattle, WA, USA

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