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chevron_left Hydrogen-deuterium exchange chevron_right
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HX-Express specifications

Information


Unique identifier OMICS_06892
Name HX-Express
Alternative name HX-Express2
Software type Package/Module
Interface Command line interface
Restrictions to use None
Operating system Unix/Linux
License GNU General Public License version 2.0
Computer skills Advanced
Version 2.0
Stability Stable
Maintained Yes

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Publications for HX-Express

HX-Express in publications

 (14)
PMCID: 5947982
PMID: 29756037
DOI: 10.1126/sciadv.aar6179

[…] a measurable difference in hdx between the 60-min time point and the equilibrium-labeled control sample., peptides exhibiting bimodal isotopic envelopes upon deuteration were further analyzed using hx-express 2.0. a brief description of the analysis procedure for peptides displaying ex1 kinetics can be found in the supplementary materials., we would like to thank j. s. mortensen and l. […]

PMCID: 5785596
PMID: 29080206
DOI: 10.1007/s13361-017-1830-9

[…] data were verified by visual inspection and exported to excel, r, or prism 6 (graphpad) for further analyses. bimodal isotopic envelopes were exported directly from masslynx (waters) and analyzed by hx-express2 []., the fraction of peptide amide hydrogens exchanged to deuterium, d f, for each peptide was calculated as:1\documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym […]

PMCID: 5072837
PMID: 27664419
DOI: 10.7554/eLife.18857.025

[…] to percent deuteration using the fully deuterated controls. confidence intervals (98%) were plotted on the δ%d plots as horizontal dashed lines. ex1 type cooperative unfolding was analyzed using hx-express2 ()., coverage maps of flid1–474(i167c/d253c) in the presence and absence of reducing agent were obtained similarly as above except for the following: 3 μl of 66 μm flid1–474(i167c/d253c) […]

PMCID: 4705694
PMID: 26420830
DOI: 10.1093/nar/gkv961

[…] in positive-ion mode. mass spectra were acquired in continuum mode over m/z 50–2000 at a mass resolution (m/δm) of approximately 20 000 (full width-half maximum). data analysis was performed using hx express software and ms tools (,)., as a control and also to determine the identities of undeuterated peptides, dnab or dnac (3 μg each, respectively) in 10 μl of buffer a supplemented with 2 mm […]

PMCID: 4480009
PMID: 26108284
DOI: 10.1038/srep11443

[…] 5 kv, cone voltage 40 v, and ion source block temperature 120 °c with a desolvation gas flow of 500 l/h at 200 °c and nebulising gas flow of 20 l/h at room temperature. the hxms data were analysed by hx-express. to identify peptic peptides, non-deuterated protein was digested and desalted as described above. the peptic peptides were eluted and collected for nanoflow-lc-ms/ms analysis, which was ca […]


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HX-Express institution(s)
Department of Medicinal Chemistry, University of Washington, Seattle, WA, USA

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