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HyLiTE specifications

Information


Unique identifier OMICS_07403
Name HyLiTE
Alternative name Hybrid Lineage Transcriptome Explorer
Software type Package/Module
Interface Command line interface
Restrictions to use None
Operating system Unix/Linux, Mac OS, Windows
Programming languages Python
License GNU General Public License version 2.0
Computer skills Advanced
Stability Stable
Maintained Yes

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Publication for Hybrid Lineage Transcriptome Explorer

HyLiTE citations

 (32)
library_books

Distinct alterations of CD68+CD163+ M2 like macrophages and myeloid derived suppressor cells in newly diagnosed primary immune thrombocytopenia with or without CR after high dose dexamethasone treatment

2018
J Transl Med
PMCID: 5833082
PMID: 29499727
DOI: 10.1186/s12967-018-1424-8

[…] washed and incubated with a cocktail of biotinylated antibodies according to the protocol provided by the manufacturer. The slides bounded with biotin were then incubated with streptavidin-conjugated Hylite Plus 555 fluor. Relative fluorescent strength was measured by LuxScan 10 K-A Microarray Scanner (CapitalBio Corporation, Beijing, China). […]

library_books

HtrA1 Mediated Intracellular Effects on Tubulin Using a Polarized RPE Disease Model

2017
PMCID: 5828370
PMID: 29269042
DOI: 10.1016/j.ebiom.2017.12.011

[…] Hylite 488 fluorescent labelled microtubules (TL488M, Cytoskeleton) were polymerized following the instructions provided by the manufacturer. In brief, monomers of the microtubules were polymerized in […]

library_books

Near infrared light decreases synaptic vulnerability to amyloid beta oligomers

2017
Sci Rep
PMCID: 5678170
PMID: 29118388
DOI: 10.1038/s41598-017-15357-x

[…] orm analysis using flow cytometry, the synaptosomes were prepared and aliquoted as described in the ELISA analysis, however, the synaptosomes were treated for 60 minutes with Aβ oligomers tagged with HyLite Fluor 488 (AnaSpec) in various concentrations ranging from 1 μM to 10 μM. The synaptosomes were then centrifuged and washed multiple times with HBK buffer and resuspended in PBS. Data was acqui […]

library_books

Strong fascin expression promotes metastasis independent of its F actin bundling activity

2017
Oncotarget
PMCID: 5746366
PMID: 29299131
DOI: 10.18632/oncotarget.22249

[…] .Analysis of MT dynamics using TIRF microscopy was performed as described previously []. Briefly, double cycled MT seeds were produced from unlabeled porcine brain tubulin, tubulin-biotin and tubulin-Hylite647 (all from Cytoskeleton) as described []. Flow chambers, assembled from glass coverslips and microscopic slides were functionalized by sequential incubation with 0.2 mg/ml PLL-PEG-biotin (Sus […]

library_books

Selective Homogeneous Assay for Circulating Endopeptidase Fibroblast Activation Protein (FAP)

2017
Sci Rep
PMCID: 5624913
PMID: 28970566
DOI: 10.1038/s41598-017-12900-8

[…] FAP cleavage site near the C-terminus of human FGF21, termed the “GP” probe, (Fig. ) was synthesized for the purpose of monitoring FAP endopeptidase activity. The peptide is flanked by a FRET-donor (HyLite Fluor 488) and a dark quencher (QXL 520). By design, fluorescence intensity is suppressed due to the close proximity of the quencher dye to the donor fluorophore, and it is liberated by proteas […]

library_books

Co delivery of paclitaxel and cetuximab by nanodiamond enhances mitotic catastrophe and tumor inhibition

2017
Sci Rep
PMCID: 5575327
PMID: 28852020
DOI: 10.1038/s41598-017-09983-8

[…] ubated with rabbit anti- p-H3S10 (1:100) antibody in PBS containing 10% FBS overnight at 4 °C, and washed three times with 0.3% Triton X-100 in PBS. Then the cells were incubated with anti-rabbit IgG-Hylite 488 (1:100) in PBS containing 10% FBS for 1 h at 37 °C, and washed three times with 0.3% Triton X-100 in PBS. The β-tubulin and nuclei were stained with the Cy3-labeled anti-β-tubulin (1:100) a […]

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HyLiTE institution(s)
Statistics and Bioinformatics Group, Institute of Fundamental Sciences, Massey University, Palmerston North, New Zealand; Institute of Mathematical and Natural Sciences, Massey University, Auckland, New Zealand

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