IHC Toolbox statistics

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Citations per year

Number of citations per year for the bioinformatics software tool IHC Toolbox

Tool usage distribution map

This map represents all the scientific publications referring to IHC Toolbox per scientific context
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Associated diseases

This word cloud represents IHC Toolbox usage per disease context

Popular tool citations

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IHC Toolbox specifications


Unique identifier OMICS_25909
Name IHC Toolbox
Alternative names Immunohistochemistry Toolbox, Immunohistochemistry (IHC) Image Analysis Toolbox
Software type Package/Module, Toolkit/Suite
Interface Graphical user interface
Restrictions to use None
Operating system Unix/Linux
Computer skills Medium
Stability Stable
Source code URL https://imagej.nih.gov/ij/plugins/ihc-toolbox/IHC_Toolbox.jar
Maintained Yes


No version available


  • person_outline Jie Shu
  • person_outline Guoping Qiu
  • person_outline Mohammad Ilyas

IHC Toolbox citations


Prion like propagation of β amyloid aggregates in the absence of APP overexpression

PMCID: 5883524
PMID: 29615128
DOI: 10.1186/s40478-018-0529-x
call_split See protocol

[…] d at 20× magnification and the percentage area covered by Aβ deposition was determined using ImageJ (http://imagej.nih.gov/ij/) and the “IHC analysis toolbox plugin” (http://imagej.nih.gov/ij/plugins/ihc-toolbox/). For each brain region, the values obtained from the three fields were averaged into a single point. […]


PPARgamma Deficiency Counteracts Thymic Senescence

Front Immunol
PMCID: 5681731
PMID: 29163553
DOI: 10.3389/fimmu.2017.01515
call_split See protocol

[…] amount Aqueous Mounting Medium (DAKO). Histological evaluation was performed with the help of Panoramic MIDI digital slide scanner (3DHistech). Image analysis was performed using ImageJ software with IHC toolbox plug-in. […]


Cucumarioside A2 2 Causes Macrophage Activation in Mouse Spleen

Mar Drugs
PMCID: 5706031
PMID: 29104230
DOI: 10.3390/md15110341
call_split See protocol

[…] e, MD, USA). The area of iba-1-, iNOS-, IL1β-positive macrophages in the white and red pulp spleen immunohistochemical staining was determined using ImageJ software (NIH, Rockville, MD, USA) (plugin: IHC Toolbox). The number of images for counting was at least 60 per group. […]


Leishmania infantum Parasites Subvert the Host Inflammatory Response through the Adenosine A2A Receptor to Promote the Establishment of Infection

Front Immunol
PMCID: 5517451
PMID: 28775724
DOI: 10.3389/fimmu.2017.00815
call_split See protocol

[…] developed using 3,3′-diaminobenzidine (Vector Laboratories). The slides were counterstained with Mayer’s hematoxylin. The areas positive for iNOS staining in the hepatic tissue were quantified using IHC Toolbox Software ImageJ (NIH, MacBiophotonics, Boston, MA, USA). The isotype control from iNOS and LY6G staining by immunohistochemistry is showed as Figure S1 in Supplementary Material. […]


Pro fibrotic compounds induce stellate cell activation, ECM remodelling and Nrf2 activation in a human 3D multicellular model of liver fibrosis

PLoS One
PMCID: 5493342
PMID: 28665955
DOI: 10.1371/journal.pone.0179995
call_split See protocol

[…] Five random areas from each immunohistochemistry (IHC) specimen were selected. Staining intensity on each image file was assessed by using the IHC Toolbox on the image analysing software NIH ImageJ (version 2.0.0-rc-56/1.51h). Two different colour segmentations were used: one recognized brown-positive cells and the other blue-positive area ( […]


Effects of Bushen Tianjing Recipe in a rat model of tripterygium glycoside induced premature ovarian failure

PMCID: 5402324
PMID: 28439292
DOI: 10.1186/s13020-017-0131-3
call_split See protocol

[…] d 3, strong staining. Scoring was performed by an experienced pathologist who was blinded to the treatment assignment using an automatic IHC image analysis software (https://imagej.nih.gov/ij/plugins/ihc-toolbox/index.html). Then, the median value across all HPFs was calculated for each animal. […]

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