IHC Toolbox statistics

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Tool usage distribution map

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Associated diseases

Associated diseases

IHC Toolbox specifications


Unique identifier OMICS_25909
Name IHC Toolbox
Alternative names Immunohistochemistry Toolbox, Immunohistochemistry (IHC) Image Analysis Toolbox
Software type Package/Module, Toolkit/Suite
Interface Graphical user interface
Restrictions to use None
Operating system Unix/Linux
Computer skills Medium
Stability Stable
Source code URL https://imagej.nih.gov/ij/plugins/ihc-toolbox/IHC_Toolbox.jar
Maintained Yes


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  • person_outline Jie Shu <>
  • person_outline Guoping Qiu <>
  • person_outline Mohammad Ilyas <>

IHC Toolbox in publications

PMCID: 5883524
PMID: 29615128
DOI: 10.1186/s40478-018-0529-x

[…] at 20× magnification and the percentage area covered by aβ deposition was determined using imagej (http://imagej.nih.gov/ij/) and the “ihc analysis toolbox plugin” (http://imagej.nih.gov/ij/plugins/ihc-toolbox/). for each brain region, the values obtained from the three fields were averaged into a single point., ten percent (wt/vol) brain homogenates were prepared in pbs and then treated […]

PMCID: 5870490
PMID: 29587836
DOI: 10.1186/s13567-018-0526-x

[…] of cells, we used the fiji® software. for each sample, on two different color images at high magnification, we defined three regions of interest (roi) of 300 × 300 pixels each. next, we used the “immunohistochemistry (ihc) image analysis toolbox” plugin in order to count automatically the number of pcna-positive nuclei that appeared in brown on each roi. the total cell number on the same roi […]

PMCID: 5859277
PMID: 29556070
DOI: 10.1038/s41598-018-23258-w

[…] digital image analysis with imagej software. quantification of immunostain colour intensity together with the percentage of area of distribution for each molecule were identified and measured by the immunohistochemistry (ihc) image analysis toolbox in the software (supplementary fig. ) followed by reciprocal immunostain calculation as previously described,., a high capacity rna-to-cdna reverse […]

PMCID: 5681731
PMID: 29163553
DOI: 10.3389/fimmu.2017.01515

[…] aqueous mounting medium (dako). histological evaluation was performed with the help of panoramic midi digital slide scanner (3dhistech). image analysis was performed using imagej software with ihc toolbox plug-in., for certain experiments, we have used wild-type and ppargamma heterozygous (haplo-insufficient) or ppargamma null (ko) mice of c57bl/6j genetic background. the mice were age […]

PMCID: 5706031
PMID: 29104230
DOI: 10.3390/md15110341

[…] md, usa). the area of iba-1-, inos-, il1β-positive macrophages in the white and red pulp spleen immunohistochemical staining was determined using imagej software (nih, rockville, md, usa) (plugin: ihc toolbox). the number of images for counting was at least 60 per group., mice were sacrificed by cervical dislocation. isolated spleens were ground with a glass homogenizer in saline solution, […]

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