Image acquisition software tools | Single plane illumination microscopy analysis
SPIM is ideally suited for capturing anatomy and patterns of gene activity in large developing specimens expressing fluorescent reporters. However, because genomes contain thousands of genes and development can last for days, it is necessary to increase the throughput of SPIM acquisitions.
An open hardware and software platform for constructing a customizable microscope for selective-plane illumination microscopy (SPIM). The OpenSPIM platform is shared with the scientific community through a public website, making light-sheet microscopy more accessible so that it can be optimized for various applications. The OpenSPIM system was intentionally designed to be simple, compact, modular and accessible.
Allows users to generate digital patterns for a wide range of illumination beams. SSPIM consists of a toolbox to produce static, spherical Gaussian, Bessel and Airy beams by simple control of a Spatial Light Modulator (SLM). It serves for making digital phase mask or amplitude mask for beam shaping. In summary, the purpose of this tool is to assist the selective plane illumination microscopy (SPIM) community to improve towards more complete and flexible open SPIM systems.
Enables high speed three-dimensional imaging of large samples at high resolution. VIsoR can achieve image acquisition of an entire mouse brain in a couple of hours with individual synaptic spines in cortical neurons visible. This method can be applied for the study of 3D structures of other biological and pathological specimen beyond brain mapping.