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Image acquisition software tools | Cryo-electron tomography analysis

Image acquisition software tools | Cryo-electron tomography analysis In cryo-electron microscopy (cryoET), a plunge frozen specimen is tilted using a rotating specimen stage inside a transmission electron microscope (TEM) and projection images are acquired onto a camera at discrete tilt angles. This series of tilted images can then be computationally reconstructed into a tomogram, i.e. the three-dimensional representation of the field of view. Collection of a tilt series is performed by iterative tracking, focusing and imaging steps for each tilt. Free and commercial software packages are available that allow fully automated acquisition of multiple tilt series.
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