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In cryo-electron microscopy (cryoET), a plunge frozen specimen is tilted using a rotating specimen stage inside a transmission electron microscope (TEM) and projection images are acquired onto a camera at discrete tilt angles. This series of tilted images can then be computationally reconstructed into a tomogram, i.e. the three-dimensional representation of the field of view. Collection of a tilt series is performed by iterative tracking, focusing and imaging steps for each tilt. Free and commercial software packages are available that allow fully automated acquisition of multiple tilt series.
(Hagen., 2017) Implementation of a cryo-electron tomography tilt-scheme optimized for high resolution subtomogram averaging. J Struct Biol.