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Traditionally, two things have made image deconvolution difficult: (1) the requirement of powerful computers and hours of computation time; and (2) the need to wade through the many algorithms—published and proprietary—for performing image deconvolution. Recent improvements in computer power such as quad processors have helped to overcome the computing power and time issues, while many references have been written to try and guide researchers through deconvolution algorithms.
(Webb., 2014) Epi-Fluorescence Microscopy. Methods Mol Biol.