Restores images from microscopic data. Huygens is based on the deconvolution approach that reassigns out-of-focus light to its origin, thus improves signal-to-noise in images. It can use physically-acquired or simulated point-spread functions (PSFs) for characterization of optical system being deconvolved. The tool shows high-performance in in-house tests on deconvolution compared to other software packages. It provides intuitive wizards for parameter selection and processing.
Serves for effective segmentation of multidimensional datasets. MIB can recognize several number of imaging formats and offers a variety of image processing tools. It also simplifies utilization and quantification of acquired data. It permits users to segment large datasets, to realize 3D visualization, and to quantify images and models. Its parameters enable users to insert plugin s to customize the program for specific needs.
Serves for correcting the intensity decay due to the photobleaching. Bleach Correction is an ImageJ plugin that works with either 2D or 3D time series. It also contains features to proceed to bleach correction. This tool is composed of three different methods: (1) the simple ratio method, (2) the exponential fitting method and (3) the histogram matching method.
Offers a collection of filters for 3D images supported by Java Native Interface. FastFilter3D performs several 3D filtering on 8-bits and 16-16-bits gray-levels stacks. This ImageJ plugin performs the following 3D filters: median, mean, minimum, maximum, maximum local and topchat.
Simplifies lattice light sheet data processing. LLSpy automates: the detection of experimental parameters from the data folder structure and metadata, and the choice of optical transfer functions (OTFs). It can conduct image corrections and manipulations, and simplifies file handling. This tool provides functions such as drag-and drop queue or visual progress indicator. It can be used with a graphical user interface or a command line interface.