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Virtual microscopy, which is the diagnostic work on completely digitized histological and cytological slides as well as blood smears, is at the stage to be implemented in routine diagnostic surgical pathology (tissue-based diagnosis) in the near future, once it has been accepted by the US Food and Drug Administration.[…] Automated extraction of content-based image information requires shading correction, constant maximum of grey values, and standardized grey value histograms.
(Kayser., 2011) Quantification of virtual slides: Approaches to analysis of content-based image information. J Pathol Inform.