Image enhancement, as front section of image process which is a very important step to better the performance of subsequent steps, has been broadly applied in many areas. There is no doubt that image enhancement has become such a significant branch that it attracts high research interest since the birth of image processing disciplines.
Refines macromolecular structures by single-particle analysis of electron cryo-microscopy (cryo-EM) data. RELION employs an empirical Bayesian approach. It can be utilized for both simulated and experimental data. This tool estimates the accuracy of alignment of individual particles and the relative contribution of the different frequencies therein. It enables the correction of per-particle defocus variations.
Permits surface rendering, multivariate statistical analysis (MSA), automatic classification, projection matching, angular reconstitution, automatic particle selection. IMAGIC can be employed for real data processing run for educational or testing purposes. This tool can exploit the properties of cheap graphic processors. It is useful for 4D analysis at atomic resolution or for refining towards multiple 3D structures.
Corrects whole frame image motion recorded with dose fractionated image stack. MotionCorr is a bioinformatic software that greatly enhances image quality and data acquisition efficiency - key bottlenecks in applying near atomic resolution electron cryomicroscopy (cryoEM) to a broad range of protein samples.
Provides an image processing environment with a particular emphasis on transmission electron microscopy (TEM) structure determination. SPARX includes a graphical user interface that provides a complete graphical programming environment with a data/process-Xow infrastructure, an extensive library of Python scripts that perform speciWc TEM-related computational tasks, and a core library of fundamental C++ image processing functions. In addition, SPARX relies on the EMAN2 library and cctbx, the open-source computational crystallography library from PHENIX. The design of the system is such that future inclusion of other image processing libraries is a straightforward task. SPARX and all dependencies are free for academic use and available with complete source.
Provides a movie alignment program. Unblur is a standalone software that evaluates per-frame shifts for each particle. The application is based on iterative alignments of each raw frame to the current best total sum of all other frames with a procedure to reduce sensitivity to noise. It also allows users to ignore filtering or to filter frame sums by the depicted exposure filter.
Analyzes, processes and visualizes multi-dimensional microscopy images. BioImageXD puts open-source computer science tools for three-dimensional visualization and analysis into the hands of all researchers, through a user-friendly graphical interface tuned to the needs of biologists. BioImageXD has no restrictive licenses or undisclosed algorithms and enables publication of precise, reproducible and modifiable workflows. It allows simple construction of processing pipelines and should enable biologists to perform challenging analyses of complex processes.
Offers a platform that allows users to make a filtering of 3D maps. bfactor also permits researchers to apply B-factors sharpening. The B-factors of a protein structure reflect the fluctuation of atoms about their average positions providing information about protein dynamics.