To improve the speed and accuracy of image correlation, image processing algorithms are developed in this work to automatically establish correspondence between images acquired by different microscopy techniques. Correlative imaging is a challenging multimodal and multiscale image registration problem. The conventional microscopy image correlation techniques require the physical addition of fiducial marks to the sample as landmarks. These landmarks can be easily identified in images captured under different modes of microscopy (e.g., SEM and fluorescence).
Solves the problem of achieving accurate association. eC-CLEM allows several types of registration, corresponding to different correlative workflows in 2D and 3D (or a mix of both dimensions). It can be used to register 3D LM (light microscopy) with 2D EM (electron microcopy), or to extract a slice with the correct transformation from an LM stack using artificial or natural fiducials. The tool offers preliminary image preprocessing for rapid data extraction. It evaluates the need to apply nonrigid registration to obtain accurate registration.
Performs 2D image registration based on elastic deformations represented by B-splines. bUnwarpJ is an ImageJ plugin that works on registration of two images, A and B: Image A is elastically deformed to look as similar as possible to image B, and, at the same time, the "inverse" transformation (from B to A) is also calculated. As a result, two images are given: the deformed versions of A and B images.
Permits users to conduct 3D modeling, image processing and measurement magnetic resonance imaging (MRI), computed tomography (CT), positron-emission tomography (PET), microscopy, scientific, and industrial imaging. 3D-DOCTOR constructs 3D surface models and volume rendering from 2D cross-section images in real time. It enables the alignment of misaligned slices in an automatic or semi-automatic manner with image alignment functions.
Registers two images and overlaids them to make correspond each position in the sample that has the same pixel. SPECREG is applicable in medical diagnosis and other applications of vibrational micro spectroscopy. It can be used to measure one and the same sample under two or more distinct types of microscopes.
Allows to work with light and electron microscopy (EM) fly brain data. Elmr supplies a solution to move between light level data and adult brain EM. It more particularly emphases on the R Neuroanatomy Toolbox package and the CATMAID web application.
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