Image signal quantification software tools | Mass spectrometry imaging analysis
Imaging by mass spectrometry has been generally regarded as a qualitative method with some recent demonstrations of quantitative analyses for small molecules. Fundamentally, the signal provided by MSI is a direct measurement of analyte relative abundance and, therefore, with the use of proper matrices and internal standards it is possible to obtain quantitative data.
Provides an easy-to-use tool for two-dimensional peptide and protein imaging of cells or tissue extracts. flexImaging provides comprehensive visualization of the spatial distribution of biomarker candidates in a tissue – thus greatly increasing the possibilities of biomarker analysis.
Assists users to analyze mass spectrometry imaging data. SCILS Lab includes a lot of functionalities allowing researchers to display multiple samples in both 2D and 3D and permitting a multitude of applications in pharmaceutical, medical, and industrial research. This software is composed of several features among which comparative analysis for uncovering discriminative m/z-markers, classification model calculation based on training data and classification of new samples, or extraction of underlying trends.
A vendor-neutral interface built on the Matlab platform designed to view and perform data analysis of Mass Spectrometry Imaging (MSI) data. A standalone version of MSiReader for which Matlab is not required is also provided. People who are unfamiliar with the Matlab language will have little difficulty navigating the user friendly interface, and users with Matlab programming experience can adapt and customize MSiReader for their own needs.
Organizes and processes multimodal mass spectrometry imaging data. HDI Software allows users to interrogate complex imaging data. The software enables the integration of multimodal imaging experiments for SYNAPT G2-Si as well as DESI imaging for Xevo G2-XS Mass Spectrometers. Some of its benefits are the image clarity and integrity, as well as Sequential sample acquisition for accelerated throughput.
A collection of R scripts for the efficient evaluation of .imzML archives in a four-step strategy: (1) calculation of the density distribution of mass-to-charge ratio (m/z) signals in the .imzML file and assembling of a pseudo-master spectrum with peak list, (2) automated generation of mass images for a defined scan range and subsequent visual inspection, (3) visualisation of individual ion distributions and export of relevant .mzML spectra and (4) creation of overlay graphics of ion images and photographies. The use of a Hue-Chroma-Luminance (HCL) colour model in MSI graphics takes into account the human perception for colours and supports the correct evaluation of signal intensities. Further, readers with colour blindness are supported. Contour maps promote the visual recognition of patterns in MSI data, which is particularly useful for noisy data sets.
Provides quantitation of target compounds (taking into account biological matrix effect) following Mass spectrometry imaging experiments. Quantinetix is a Quantitative Imaging Mass Spectrometry Software which offers normalization to get “real images” and provides concentration of target compounds. It is specially designed to ADMET study, PK/PD study, Toxicity study, In support of Whole Body Autoradiography and Proteomics and Lipidomics studies.
An open source web- and MATLAB-based software tool for both DESI and MALDI mass spectrometry imaging (MSI) that performs computationally intensive functions on a remote server. These functions include converting data from a variety of file formats into a common format easily manipulated in MATLAB, transforming time-series mass spectra into mass spectrometry images based on a probe spatial raster path, and multivariate analysis. OmniSpect provides an extensible suite of tools to meet the computational requirements needed for visualizing open and proprietary format MSI data.