bookmark SuReSim / Super Resolution Simulation A software tool that simulates localization data of arbitrary three-dimensional structures represented by ground truth models, allowing users to systematically explore how changing experimental parameters can affect potential imaging outcomes. SuReSim can generate simulated localizations from ground truth models in two alternative modes. Direct simulation (left) provides instant 3D visualization, allowing one to explore a structure and predict whether it can be resolved with SMLM. Furthermore, localization files and rendered images can be used for postprocessing of the data. The indirect approach (right) produces raw SMLM data in the form of image stacks. These can be analyzed with any SMLM reconstruction software. The reconstructed data can also be compared to ground truth data to validate the reconstruction software.