Image5D statistics

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Citations per year

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Popular tool citations

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Associated diseases

Associated diseases


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Image5D specifications


Unique identifier OMICS_25926
Name Image5D
Software type Package/Module
Interface Graphical user interface
Restrictions to use None
Operating system Windows
Programming languages Java
Computer skills Medium
Version 1.1.6
Stability Stable
Maintained Yes



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  • person_outline Joachim Walter <>

Additional information

Image5D in pipeline

PMCID: 2635477
PMID: 18817522
DOI: 10.1111/j.1600-0854.2008.00815.x

[…] and registered using the ‘rigid body rotation’ function of turboreg () to avoid rotational differences. merge of aligned/registered multichannel flm image and grayscale tem image was performed using image5d plug-in. 3d fluorescence volume rendering was obtained combining two plug-ins developed in our laboratory: ‘mesh maker microscobioj’ implemented the marching cube () algorithm to extract […]

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Image5D in publications

PMCID: 5591248
PMID: 28887440
DOI: 10.1038/s41598-017-10059-w

[…] in parallel by light microscopy. the pictures obtained were processed using fiji ( deltavision files were first adjusted for brightness and contrast and then prepared using the “image5d z/tp montage function”. afterwards, a non-destructive scale bar was added to all slices. the time-stamper plug-in allowed labeling of observation time for all slices. finally, the movies […]

PMCID: 5063964
PMID: 27694947
DOI: 10.1038/ncomms13010

[…] and manually inspected as to the overlap of orco and cd8 channels., to quantify orco (red) and mcd8-gfp (blue) co-staining with gfp fluorescence (green) in the larvae (), we used imagej with the image5d plugin and zen zeiss software to mark and count cells in each of the three fluorescence channels., to quantify the overlap of orco staining (red) and endogenous gfp fluorescence (green) […]

PMCID: 4895443
PMID: 27249668
DOI: 10.1038/ncomms11643

[…] , , , , , , . zeiss lsm 710 was used for capturing movies with higher temporal resolution using acquisition speed of two frames per minute and played at a rate of 6 frames per second for , , , . the image5d plug-in from imagej was used for image processing and analysis of live movies., imaging conditions were kept uniform across most of the experiments. occasionally, pmt gains were slightly […]

PMCID: 4747903
PMID: 26833089
DOI: 10.1016/j.molcel.2016.01.001

[…] 25th) per experiment. per condition we plot the average of this signal as taken from two independent biological replicates., fish was performed as described previously and detailed in the . imagej (image5d plugin) software was used to score touching (no unstained pixel in between fish signals) and nontouching (with unstained pixel(s) in between) alleles., p.j.w., p.h.l.k., and y.z. performed […]

PMCID: 4647693
PMID: 26573515
DOI: 10.1186/s12915-015-0205-6

[…] proliferating on the slide for 40–60 min in a 30 °c incubator prior to imaging., to quantify gfp intensity at each time point, we first used imagej ( [] and its plugin image5d ( to average across each z-stack and flatten it to 2d. gfp intensity was then quantified using matlab (the mathworks inc.) code previously […]

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