ImageMaster 2D protocols

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ImageMaster 2D specifications

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Unique identifier OMICS_02542
Name ImageMaster 2D
Software type Package/Module
Interface Graphical user interface
Restrictions to use License purchase required
Operating system Windows
License Commercial
Computer skills Medium
Stability Stable
Maintained Yes

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ImageMaster 2D in pipeline

2017
PMCID: 5376303
PMID: 28362854
DOI: 10.1371/journal.pone.0174885

[…] refrigerated conditions. after that, fungal proteins were stained with coomassie brilliant blue (cbb) and then gel images were acquired and analysed by using the imagescanner iii (ge healthcare) and imagemaster 2d platinum software (ge healthcare), respectively., the protein spots most differentially expressed were manually excised and submitted for protein identification by lc-ms/ms, performed […]


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ImageMaster 2D in publications

 (308)
PMCID: 5943403
PMID: 29760951
DOI: 10.1038/s41420-018-0053-6

[…] protein bands were visualized by supersignal west pico chemiluminescence substrate (pierce biotechnology, inc., rockford, il) and autoradiography. band intensity data was obtained using imagemaster 2d platinum version 6.0 (ge healthcare),., the non-transfected, sicontrol-transfected, sihsp90-transfected, and silamin a/c-transfected dpcs were grown in each of 6-well plate […]

PMCID: 5902686
PMID: 29692790
DOI: 10.3389/fpls.2018.00425

[…] mm dithiothreitol (dtt) and 0.5% immobilized ph gradient (ipg) buffer) were loaded onto an 18 cm linear gradient ipg strip (ge healthcare, little chalfont, united kingdom) and separated by 2-de. the imagemaster 2d platinum 7.0 (ge healthcare, united states) was used to analyze the images and only those with significant and biological reproducible changes (abundance variation at least two-fold, […]

PMCID: 5869912
PMID: 29616196
DOI: 10.3389/fcimb.2018.00085

[…] volume to the total spot's volume on the gel. a student's t-test (p < 0.05) and a threshold of 1.5-fold change in the expression spot were utilized in the data analysis., typhoon scanne and imagemaster 2d platinum 5.0 were employed to collect the protein point position coordinates and expression data. the phosphoprotein spots of the wt and δstk were compared, and the wt-specific spots […]

PMCID: 5843658
PMID: 29520092
DOI: 10.1038/s41598-018-22709-8

[…] (ge healthcare) and the silver staining kit (ge healthcare). stained gels were digitalized using an imagescanner iii and the labscantm software (v6.0, ge healthcare). images were processed using the imagemaster 2d platinum software for protein spots enumeration., two groups of five balb/c mice each were inoculated through the intraperitoneal (i.p.) route with 5 × 106 yeast cells of s. […]

PMCID: 5835051
PMID: 29535716
DOI: 10.3389/fimmu.2018.00316

[…] were stained with deep purple protein fluorescence dye (ge healthcare) and visualized by using typhoon 9200 laser scanner (ge healthcare)., protein spots visualized in 2-de gels were analyzed using imagemaster 2d platinum software (ge healthcare). parameters used for spot detection were (i) minimal area = 10 pixels; smooth factor = 2.0 and (ii) saliency = 200. a reference gel was created […]


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