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ImageMaster 2D specifications

Information


Unique identifier OMICS_02542
Name ImageMaster 2D
Software type Package/Module
Interface Graphical user interface
Restrictions to use License purchase required
Operating system Windows
License Commercial
Computer skills Medium
Stability Stable
Maintained Yes

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No version available

ImageMaster 2D citations

 (52)
library_books

Proteomic identification of moesin upon exposure to acrolein

2018
Proteome Sci
PMCID: 5773073
PMID: 29375273
DOI: 10.1186/s12953-017-0130-4

[…] t Blue G-250 according to the manufacturer’s instructions. The 2-D gels were scanned with an ImageScanner (Amersham Biosciences) in transmission mode. Spot detection and matching were performed using ImageMaster 2D version 5.0 (Amersham Biosciences). Digitized images were analyzed using the ImageMaster program to calculate the 2-D spot intensity by integrating the optical density over the spot are […]

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Variety and Dynamics of Proteoforms in the Human Proteome: Aspects of Markers for Hepatocellular Carcinoma

2017
Proteomes
PMCID: 5748568
PMID: 29168748
DOI: 10.3390/proteomes5040033
call_split See protocol

[…] . All procedures for 2DE were carried out as described previously [,,]. Gels were stained with Coomassie Blue R350, scanned by ImageScanner III (GE Healthcare, Pittsburgh, PA, USA) and analysed using ImageMaster 2D Platinum 7.0 (GE Healthcare) []. The tryptic peptides from crude lysates for MS analysis were obtained using a protocol for filter-aided sample preparation (FASP) []. Mass spectrometry […]

library_books

Persistent Unresolved Inflammation in the Mecp2 308 Female Mutated Mouse Model of Rett Syndrome

2017
PMCID: 5448068
PMID: 28592917
DOI: 10.1155/2017/9467819

[…] Gel imaging was performed by using ImageMaster 2D Platinum v7.0 software (GE Healthcare). A reference gel for each group was defined for the comparative analyses. The background was subtracted from all gels using the average on-boundar […]

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Roles of the Putative Type IV like Secretion System Key Component VirD4 and PrsA in Pathogenesis of Streptococcus suis Type 2

2016
PMCID: 5133265
PMID: 27995095
DOI: 10.3389/fcimb.2016.00172
call_split See protocol

[…] entification of proteins by mass spectrometry (Bruker Dalton, Ultraflex III TOF/TOF) were performed as described elsewhere (Jing et al., ). Comparison of secreted protein spots was performed with the ImageMaster 2D platinum 5.0 (GE Healthcare, Uppsala, Sweden). Only spots with relative high abundance showing consistent and reproducible changes (>3 folds) in 2D-gels were excised and subjected to ma […]

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Overexpression of a Pathogenesis Related Protein 10 Enhances Biotic and Abiotic Stress Tolerance in Rice

2016
PMCID: 5117864
PMID: 27904462
DOI: 10.5423/PPJ.OA.06.2016.0141
call_split See protocol

[…] and the gels were stained with Coomassie brilliant blue G-250 (CBB). The stained gels were scanned using transmissive scanner (Powerlook III; UMAX, Fort Worth, TX, USA), and images were analyzed with ImageMaster 2D Platinum software 6.0 (Amersham Biosciences AB, Uppsala, Sweden). Protein spots were excised from CBB-stained gels, digested with trypsin, and extracted with method describes previously […]

library_books

Proteomic Comparison of Two Dimensional Gel Electrophoresis Profiles from Human Lung Squamous Carcinoma and Normal Bronchial Epithelial Tissues

2016
PMCID: 5172349
PMID: 15626334
DOI: 10.1016/S1672-0229(03)01008-8

[…] Imagescanner (Amersham Pharmacia Biotech). The spot-intensity calibration, spot detection, background abstraction, matching, 1-D calibration, and the establishment of average-gel were performed with ImageMaster 2D Elite 4.01analysis software (Amersham Pharmacia Biotech). The reproducibility of spot position was calculated according to Gorbett’s method . Statistical analysis was carried out with S […]

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ImageMaster 2D institution(s)
Informer Technologies, Inc.

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