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Protocols

ImageQuest specifications

Information


Unique identifier OMICS_05665
Name ImageQuest
Software type Toolkit/Suite
Interface Graphical user interface
Restrictions to use License purchase required
Operating system Unix/Linux
License Commercial
Computer skills Medium
Stability No
Maintained No

Versioning


No version available

ImageQuest citations

 (28)
library_books

Visualization of Mycobacterial Biomarkers and Tuberculosis Drugs in Infected Tissue by MALDI MS Imaging

2018
Anal Chem
PMCID: 5956283
PMID: 29668262
DOI: 10.1021/acs.analchem.8b00985

[…] ere acquired in negative polarity across the mass range of m/z 500–2000 with a laser energy of 20 μJ and five shots per position (one microscan per position).2D ion images were generated using Thermo ImageQuest software (v1.01). Normalized ion images of RIF were generated by dividing the RIF [M – H]− signal (m/z 821.397 ± 0.003) by the RIF-D3 [M – H]− signal (m/z 824.416 ± 0.003). Normalized ion i […]

library_books

Spatial temporal profiling of prodiginines and serratamolides produced by endophytic Serratia marcescens harbored in Maytenus serrata

2018
Sci Rep
PMCID: 5869619
PMID: 29588473
DOI: 10.1038/s41598-018-23538-5

[…] rgy collisional dissociation (HCD) with 45 eV and a resolution of 35 000 @ m/z 200. Data processing and mapping of mass pixels corresponding to the target compounds was done with the software package ImageQuest (v. 1.1.0; Thermo Fisher Scientific, Germany). Ion images were generated with a bin width of ± 2.0 ppm for full scans and ± 3.0 ppm for MS2 experiments. The mass pixels are shown as false c […]

call_split

Extracellular N acetylaspartylglutamate released in the nucleus accumbens modulates the pain sensation: Analysis using a microdialysis/mass spectrometry integrated system

2018
PMCID: 5813845
PMID: 29310499
DOI: 10.1177/1744806918754934
call_split See protocol

[…] signals of DPP-DA (m/z 368→232) and DPP-DA-d4 (m/z 372→232) were monitored with a precursor ion isolation width of 1.0 m/z units. The obtained spectral data were then transformed to image data using ImageQuest 1.0.1 software (Thermo Fisher Scientific). Note that the DA signal was normalized by the IS (DA-d4) signal simultaneously obtained from the same section. […]

library_books

3D MALDI Mass Spectrometry Imaging of a Single Cell: Spatial Mapping of Lipids in the Embryonic Development of Zebrafish

2017
Sci Rep
PMCID: 5668422
PMID: 29097697
DOI: 10.1038/s41598-017-14949-x

[…] e mode using an Orbitrap mass analyzer (resolution of 30,000 at m/z 400) for m/z scan range of 100–1000. Raw mass spectral files acquired from the Orbitrap analyzer were used to generate images using ImageQuest software (Thermo Scientific; San Jose, CA, USA) with a mass window of ±0.003 Da and without normalization. Compound identification was based on accurate mass and confirmed by MS/MS analysis […]

library_books

Unraveling Drug Penetration of Echinocandin Antifungals at the Site of Infection in an Intra abdominal Abscess Model

2017
Antimicrob Agents Chemother
PMCID: 5610477
PMID: 28739797
DOI: 10.1128/AAC.01009-17

[…] a size at which small necrotic areas within lesions could easily be resolved, and no overlapping of the laser spot on adjacent acquisitions was observed.Data visualization was performed using Thermo ImageQuest software. Normalized ion images of CD101 were generated by dividing CD101 [M+H]+ signal (m/z 1,225.603 ± 0.005) by CD101-D9 [M+H]+ signal (m/z 1,234.651 ± 0.005). Normalized ion images of m […]

library_books

Ethambutol Partitioning in Tuberculous Pulmonary Lesions Explains Its Clinical Efficacy

2017
Antimicrob Agents Chemother
PMCID: 5571334
PMID: 28696241
DOI: 10.1128/AAC.00924-17

[…] of 1 microscan per position). The laser step size was set at 50 μm, which enabled small necrotic areas within lesions to be resolved without overlap of the laser spot on adjacent acquisitions. Thermo ImageQuest software (version 1.01) was used to reconstruct two-dimensional (2D) ion images. Normalized ion images of EMB were generated by dividing EMB [M+H]+ signal (m/z 205.193 ± 0.003) by EMB-d10 [ […]


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ImageQuest institution(s)
ThermoFisher Scientific

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