ImFCS specifications


Unique identifier OMICS_25904
Name ImFCS
Software type Package/Module
Interface Graphical user interface
Restrictions to use None
Input format TIFF
Operating system Windows
Programming languages C++
Computer skills Medium
Version 1.491
Stability Stable
ImageJ, Imagescience, Apache Poi
Maintained Yes



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  • person_outline Thorsten Wohland <>

Publication for ImFCS

ImFCS in publications

PMCID: 5688065
PMID: 29142280
DOI: 10.1038/s41598-017-15821-8

[…] analyze hsp levels following heat treatment at different temperatures (fig. , supplementary fig. ). to be able to compare the results with image-based fluorescence correlation spectroscopy (imfcs) studies (fig. ), we employed a cho cell line expressing glycosylphosphatidyl-inositol-anchored monomeric green fluorescent protein (gpi-mgfp). out of the five major hsps examined, only hsp25, […]

PMCID: 5586345
PMID: 28904870
DOI: 10.1002/2211-5463.12285

[…] diffusion of resting and inlb‐bound met within the plasma membrane of living cells using single‐particle tracking following the upaint protocol and imaging fluorescence correlation spectroscopy (imfcs) , . both methods apply tirf illumination, which allows selective excitation of labeled ligands bound to receptors at the basal cell membrane under exclusion of background fluorescence […]

PMCID: 5599997
PMID: 28626941
DOI: 10.1111/febs.14139

[…] fps for wnt3‐egfp and dii‐c18 measurements, respectively. after image acquisition, the data were analyzed by a home‐written plugin (imaging_fcs 1.491, available at for imagej (imagej). autocorrelation analysis on the fluorescence fluctuations of each pixel was performed and the raw autocorrelation functions (acf) were fitted […]

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ImFCS institution(s)
Singapore-MIT Alliance, National University of Singapore (NUS), Singapore, Singapore; Department of Chemistry, NUS, Singapore, Singapore; Bioinformatics Institute, Singapore, Singapore; Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany
ImFCS funding source(s)
Supported by the Alexander von Humboldt Foundation, a grant from the Ministry of Education of Singapore (R-143-000-358-112), and a scholarship of the Singapore-MIT Alliance.

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