Predicts the constitution of ten immune cells from the RNASeq data of mouse tissues. Seq-ImmuCC is a computational model that depicts the constitution of major immune cells across different tissues or organs through the mouse transcriptomal data. It can provide a cell-centric view for transcriptomal data to monitor tissue infiltrating immune cells under various conditions. The program was validated in large and various types of independent datasets, including simulated data, public data and experimental data.
Allows users to reconstruct the native T-cell receptors (TCR)αβ from single cell RNA-seq data of Ag-specific T cells and to link these with the gene expression profile of individual cells. VDJPuzzle enables analysis about TCR diversity and its relationship with the transcriptional profile of different clones. Moreover, single-cell transcriptome analysis can successfully distinguish Ag-specific T cell populations sorted directly from resting memory cells in peripheral blood and sorted after ex vivo stimulation. Moreover, it has been adapted for B-cell receptor (BCRs) and includes additional features to reliably characterizes somatic hypermutation (SHMs).
Allows investigators to use scRNA-seq for assembling BCR sequences at single cell resolution. BASIC performs semi-de novo assembly in two stages: (i) uses known constant and variable regions to identify anchor sequences, and (ii) uses these anchors to guide the de novo assembly of the BCR. BASIC can help couple gene expression information from scRNA-seq with immune repertoire, and facilitate studies between B-cell receptor features, clonality, differentiation and transcriptional programming at single cell resolution.
Rebuilds paired full-length B-cell receptor (BCR) sequences. BraCeR is a program which can be used for downstream analyses. This program is able to reconstitute multiple heavy and light chains detected within a target cell as well as to highlight non-productively rearranged chains. This program can also be used as a method for deducing clonal relationships and perform immunoglobulin lineage reconstruction.
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