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RUCS / Rapid identification of PCR primers for Unique Core Sequences
Eases the burden of Polymerase chain reaction (PCR) primer design. RUCS is a web application that allows users to (i) identify unique core sequences for a given dataset of positive and negative genomes, and (ii) combines the primer pair prediction of Primer3, with a novel in silico PCR product validation method. In this condition, RUCS can predict amplicons for PCR primer pairs against a positive and a negative set of references.
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