In-Silico PCR specifications
Information
| Unique identifier | OMICS_02344 |
|---|---|
| Name | In-Silico PCR |
| Interface | Web user interface |
| Restrictions to use | None |
| Output data | A file containing all sequence in the database that lie between and include the primer pair. |
| Output format | FASTA |
| Computer skills | Basic |
| Stability | Stable |
| Maintained | Yes |
Documentation
Maintainers
- person_outline UCSC Genome Informatics Group <>
- person_outline Jim Kent <>
In-Silico PCR citations
(2)Genome Sequencing and Comparative Genomics of the Broad Host-Range Pathogen Rhizoctonia solani AG8
[…] primer pairs were designed from coding-exon sequences (cds) using primer3 [100] (opt. amplicon 200 bp, primer 18–25 bp, opt. tm 60°c, max. δtm 1°c, min. gc clamp 2 bp, max. homopolymer 3 bp). in silico pcr screening via e-pcr [101] required ≤1 amplicon (10 bp to 10 kbp) versus genome assembly and cds sequences. quantitative pcr was performed with 2 technical replicates and 3 biological […]
Fine-Mapping, Gene Expression and Splicing Analysis of the Disease Associated LRRK2 Locus
[…] 2step rt-pcr kit (qiagen, uk). all primers for this analysis were designed using the primer3 software (fokker.wi.mit.edu/primer3/input.htm), and then they were blast searched against ucsc human. in-silico pcr tools. an aliquot of total rna from 48 samples, originating from four brain regions (occipital cortex, substantia nigra, medulla and cerebellum) and 12 individuals were used as a subset […]
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