Allows for the computational identification of transposon insertion sites in known bacterial genome sequences after transposon mutagenesis experiments. The used approach is based on the observation that restriction endonucleases digestions of bacterial DNA yields unique pattern of DNA fragments with defined sizes. Transposon insertion changes the size of the hosting DNA fragment by a known number of base pairs. The exact sizes of this fragment can be determinate by Southern blot hybridization and identified with subsequent computational analysis.