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Intron retention identification software tools | RNA sequencing data analysis

Alternative splicing (AS) affects up to 95% of multi-exonic genes in humans (Pan et al., 2008). The three main types of AS are exon skipping, alternative 5′ or 3′ usage and intron retention (IR). IR occurs when an intron is transcribed into pre-mRNA and remains in the final mRNA. It constitutes a class of AS that is often neglected because these events are difficult to measure reliably.

Reference:
(Pan et al., 2008) Deep surveying of alternative splicing complexity in the human transcriptome by high-throughput sequencing. Nat Genet.

Source text:
(Middleton et al., 2017) IRFinder: assessing the impact of intron retention on mammalian gene expression. Genome Biology.

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