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Highly variable contractile performance correlates with myocyte content in trabeculae from failing human hearts
[…] ethods with custom written scripts in Python software. In brief, RyR-JPH2 dual-labelled images were thresholded using the mode plus standard deviation value in ImageJ, while JPH2-α-tubulin images had isodata thresholding applied and masks were generated of each protein label with Python software. From each protein label, the distance to the nearest labelling of the second protein mask was determin […]
Comparing Pixel and Object Based Approaches in Effectively Classifying Wetland Dominated Landscapes
[…] y area draining into Lake Baikal, a United Nations Educational, Scientific, and Cultural (“UNESCO”) World Heritage Site located in Siberian Russia. We furthermore compared the efficacy of parametric (ISODATA, ML) and non-parametric (RF) approaches and iteratively analyzed outputs with spatial and spectral classification metrics seeking a parsimonious and effective wetland classification solution. […]
Automated Ischemic Lesion Segmentation in MRI Mouse Brain Data after Transient Middle Cerebral Artery Occlusion
[…] an-Zadeh et al., ; Wang et al., ), our algorithm operates on a single contrast (T2) and does not require a pre-scan.Unlike other methods developed for small animal data (HRS by Ghosh et al., , , and ISODATA by Jacobs et al., ,, , ,), the ischemic lesion segmentation approach presented here only makes use of the quantitative T2 maps. Contrary to T2-weighted MRI data, true quantitative MRI maps (T2 […]
Identification of lesion subtypes in biopsies of ductal carcinoma in situ of the breast using biomarker ratio imaging microscopy
[…] h Metamorph and processed with MetaFluor software (Molecular Devices, Downingtown, PA) to calculate image ratios. Micrographs were evaluated using ImageJ software. Segmentation was performed with the ISODATA (iterative self-organizing data analysis) technique. A local implementation of the Otsu algorithm was used for comparison. (Global segmentation is recommended over local implementations of thr […]
Retinal Remodeling and Metabolic Alterations in Human AMD
[…] s of small-molecule signatures as those in the primate, cat, rabbit and rat retinas (Fletcher and Kalloniatis, ; Kalloniatis et al., ; Marc et al., ; Marc, ). Classifying all the data with k-means or ISODATA clustering produces raw theme maps, which are then converted to refined theme maps superimposed on a single reference channel. Theme maps and rgb displays simplify tracking of 10 major cell ph […]
An Improved STARFM with Help of an Unmixing Based Method to Generate High Spatial and Temporal Resolution Remote Sensing Data in Complex Heterogeneous Regions
[…] The ISODATA algorithm was applied to cluster a Landsat image collected August 19, generating land cover component maps with class number (k) as [,,,,,]. Two kinds of window scales which have pronounced im […]
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