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IsoDesign specifications


Unique identifier OMICS_06434
Name IsoDesign
Software type Package/Module
Interface Command line interface
Restrictions to use None
Operating system Unix/Linux
Computer skills Advanced
Stability Stable
Maintained Yes


No version available


  • person_outline Jean-Charles Portais

Publication for IsoDesign

IsoDesign citations


Multilevel interaction of the DnaK/DnaJ(HSP70/HSP40) stress responsive chaperone machine with the central metabolism

Sci Rep
PMCID: 5269706
PMID: 28128357
DOI: 10.1038/srep41341

[…] glucose labeled mixture at 30 °C to minimize the effect of unlabeled carbon. Note that the ratio of (1-13C) and (U-13C) glucose used in this experiment was determined and optimized using the software Isodesign.Culture samples were collected for NMR analyses at an OD600 of 1 to ensure both isotopic and metabolic steady-state conditions. In this case, 9 ml of solvent H2O/Methanol/Acetonitrile (20:40 […]


Investigation of useful carbon tracers for 13C metabolic flux analysis of Escherichia coli by considering five experimentally determined flux distributions

Metab Eng Com
PMCID: 5678827
PMID: 29142823
DOI: 10.1016/j.meteno.2016.06.001

[…] re of [1-13C] and [U-13C]glucose at 8:2, the best carbon tracer for the estimated metabolic flux distribution should be investigated by employing optimization methods such as EMU basis vectors () and Isodesign (), when more precision is needed in the flux estimation of the anaplerotic and the glyoxylate pathways. Precise flux estimation would be attained by the second 13C-MFA employing an optimize […]

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IsoDesign institution(s)
MetaSys, Université de Toulouse, Toulouse, France

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