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True equilibrium measurement of transcription factor DNA binding affinities using automated polarization microscopy
[…] measurements. Under the additivity assumption, one needs to only determine the KD of the consensus sequence and of each single base mutation of the consensus. The PWM can then be constructed by,7pb=1KDb∑b∈A,C,G,T1KDbwhere pb is the (inferred) probability of nucleotide b at a particular sequence position and KDb the KD measured for the corresponding mutation. The specificity of a PWM can be summar […]
Lamina depth and thickness correlate with glaucoma severity
[…] software which was provided from Heidelberg SD-OCT. Interobserver reliability (as assessed by intraclass correlation coefficient [ICC]) was measured for LD and LT using two independent observers (MK, KDB) for a subset of the subjects.Comparisons between NTG and HTG groups were performed using the independent t-test for continuous variables or Chi-square test for categorical variables. The relation […]
Isolation of cellulolytic bacteria from the intestine of Diatraea saccharalis larvae and evaluation of their capacity to degrade sugarcane biomass
[…] ions. A sequence from a Chlamydia pneumoniae strain CHT16SR (L06108) was used to root the tree. The resulting 16S rDNA gene sequences for the isolates Klebsiella oxytoca Kd70 TUC-EEAOC, K. pneumoniae KdB5 TUC-EEAOC, K. variicola KdB1 TUC-EEAOC, Stenotrophomonas maltophilia Kd3 TUC-EEAOC, S. rhizophila Kd46 TUC-EEAOC, Bacillus pumilus Kd109 TUC-EEAOC, Enterococcus casseliflavus Kd7 TUC-EEAOC, Micro […]
Quantitative assessment of minimal effective concentration of erythropoiesis stimulating agents
[…] ucer constant in the operational model of agonism and serves as a marker of efficacy. Equation 7 can be rewritten as: In the case when endogenous EPO level is far less than that for exogenous EPO (CB/KDB << CA/KDA), equation 9 can be simplified to: As , the maximal effect (α) to a given agonist can be defined as: C50, which represents the concentration of agonist that produces the half-maximal eff […]
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